Fraser I, Hughes D, Gordon S
Sir William Dunn School of Pathology, University of Oxford, UK.
Nature. 1993 Jul 22;364(6435):343-6. doi: 10.1038/364343a0.
Macrophages interact with other cells and components of the extracellular environment by means of adhesion receptors. Adhesion to artificial substrata in vitro facilitates isolation of macrophages, and has been used to generate antibodies that inhibit their migration in vivo. Unlike other cell types, macrophages attach to tissue culture plastic in the absence of divalent cations. Here we use an adhesion assay exploiting this property to isolate a rat monoclonal antibody, 2F8, which totally inhibits divalent cation-independent adhesion of murine macrophages to tissue culture plastic in the presence of fetal calf serum. Immunoprecipitation from macrophages and stably transfected Chinese hamster ovary cells revealed that the antigen recognized by monoclonal 2F8 is identical to murine macrophage scavenger receptor. We propose a novel function for this molecule, previously described as an endocytic receptor, thus providing a mechanism for mononuclear phagocyte recruitment to and retention in ligand-rich tissues such as in atherosclerotic lesions.
巨噬细胞通过黏附受体与细胞外环境中的其他细胞及成分相互作用。体外黏附于人工基质有助于巨噬细胞的分离，并且已被用于生成抑制其体内迁移的抗体。与其他细胞类型不同，巨噬细胞在没有二价阳离子的情况下可附着于组织培养塑料。在此，我们利用这一特性进行黏附测定，以分离出一种大鼠单克隆抗体2F8，该抗体在胎牛血清存在的情况下能完全抑制小鼠巨噬细胞与组织培养塑料的非二价阳离子依赖性黏附。对巨噬细胞和稳定转染的中国仓鼠卵巢细胞进行免疫沉淀分析表明，单克隆抗体2F8识别的抗原与小鼠巨噬细胞清道夫受体相同。我们为该分子提出了一种新功能，该分子先前被描述为一种内吞受体，从而为单核吞噬细胞募集到富含配体的组织（如动脉粥样硬化病变组织）并在其中滞留提供了一种机制。
