Larson P J, Thorp J M, Miller R C, Hoffman M
Department of Obstetrics and Gynecology, University of North Carolina School of Medicine, Chapel Hill 27599-7570, USA.
Am J Perinatol. 1995 May;12(3):157-60. doi: 10.1055/s-2007-994440.
To test the noninvasive monocyte monolayer assay in predicting hemolytic severity in utero, we studied 18 patients from two institutions with significant erythrocyte alloantibodies. Serum samples were obtained from each patient. Each subject donated a serum sample during her pregnancy. Monocytes were harvested from a single healthy donor and grown in monolayer culture. Erythrocytes with the appropriate antigens were sensitized with maternal serum and incubated with the monocyte monolayers. Erythrophagocytosis was scored as a percentage of the positive control. Perinatal outcomes were assessed post hoc and cases were classified as unaffected or mildly, moderately, or severely affected using standard definitions. Prenatal management was conducted without knowledge of the results. Six of the 18 patients had severe disease and one of the 18 had moderately severe disease. At a cutoff level of 20, the assay generated the following results: sensitivity was 7 of 7 (100%), specificity was 10 of 11 (90.9%), positive predictive value was 7 of 8 (87.5%), and negative predictive value was 10 of 10 (100%). The monocyte monolayer assay appears to be a useful, noninvasive modality for predicting the severity of hemolytic disease in utero.
为了测试非侵入性单核细胞单层试验在预测宫内溶血严重程度方面的效果，我们研究了来自两家机构的18例有显著红细胞同种抗体的患者。从每位患者获取血清样本。每位受试者在孕期捐献一份血清样本。从一名健康供体采集单核细胞并在单层培养中生长。带有相应抗原的红细胞用母体血清致敏后与单核细胞单层共同孵育。红细胞吞噬作用按阳性对照的百分比进行评分。围产期结局在事后进行评估，病例根据标准定义分为未受影响、轻度、中度或重度受影响。产前管理在不知道结果的情况下进行。18例患者中有6例患有严重疾病，18例中有1例患有中度严重疾病。在临界值为20时，该试验产生了以下结果：灵敏度为7/7（100%），特异性为10/11（90.9%），阳性预测值为7/8（87.5%），阴性预测值为10/10（100%）。单核细胞单层试验似乎是一种用于预测宫内溶血病严重程度的有用的非侵入性方法。
