Dhingra-Kumar N, Duguid J K, Bromilow I M
Mersey & North Wales Regional Blood Transfusion Centre, Liverpool, UK.
Indian J Med Res. 1995 Aug;102:71-6.
The present study was undertaken to estimate the predictive value of antibody titration, antibody quantitation and monocyte monolayer assay (MMA) in assessment of severity of haemolytic disease of the newborn (HDN). Serum samples from 45 alloimmunized mothers, with anti-D(23), anti-c(10), anti-K(6), anti-E(5) and anti-e(1) were taken for the study. The results obtained were compared and the efficiency of each technique in predicting the severity of HDN was assessed. Antibody quantitation and MMA (phagocytic index) correlated well with severity of HDN in mothers with anti-D antibodies. Antibody quantitation (anti-D) had a positive predictive value of 54.5 per cent and negative predictive value of 85.7 per cent while MMA had a positive predictive value of 75 per cent and a negative predictive value of 100 per cent. These findings suggest MMA to be a good negative predictor of HDN but not a good positive predictor of haemolytic disease of the newborn.
本研究旨在评估抗体滴定、抗体定量和单核细胞单层试验（MMA）在评估新生儿溶血病（HDN）严重程度方面的预测价值。采集了45例同种免疫母亲的血清样本进行研究，这些母亲分别具有抗-D（23例）、抗-c（10例）、抗-K（6例）、抗-E（5例）和抗-e（1例）。对所得结果进行比较，并评估每种技术在预测HDN严重程度方面的效率。在具有抗-D抗体的母亲中，抗体定量和MMA（吞噬指数）与HDN的严重程度密切相关。抗体定量（抗-D）的阳性预测值为54.5%，阴性预测值为85.7%，而MMA的阳性预测值为75%，阴性预测值为100%。这些发现表明，MMA是HDN的良好阴性预测指标，但不是新生儿溶血病的良好阳性预测指标。
