Durkin W J, Ghanta V K, Balch C M, Davis D W, Hiramoto R N
Cancer Res. 1979 Feb;39(2 Pt 1):402-7.
Tumor cells from animals and humans were treated with drugs under tissue culture conditions. Tumor cells from the sensitive L1210 model were studied first. A dose-response curve was derived between drug exposure and subsequent cytotoxicity in L1210. The concentration of drug and duration of exposure were factors critical to the subsequent development of in vitro cytotoxicity. The in vitro dosage which effected 50% leukemic cell death in L1210 cells correlated with reported in vivo drug levels. Other tumor models and human neoplastic cells were studied at this dosage level. A good correlation was noted in these studies between the in vivo responsiveness and the in vitro chemotherapy results in both animals and humans. It was suggested by these results that it may be possible to predict cancericidal drug activity for individual neoplasms by assaying the tumor cells in vitro for drug sensitivity.
在组织培养条件下，用药物处理来自动物和人类的肿瘤细胞。首先研究了来自敏感L1210模型的肿瘤细胞。得出了L1210中药物暴露与后续细胞毒性之间的剂量反应曲线。药物浓度和暴露持续时间是体外细胞毒性后续发展的关键因素。在L1210细胞中导致50%白血病细胞死亡的体外剂量与报道的体内药物水平相关。在这个剂量水平上研究了其他肿瘤模型和人类肿瘤细胞。在这些研究中发现，动物和人类的体内反应性与体外化疗结果之间有良好的相关性。这些结果表明，通过体外检测肿瘤细胞的药物敏感性，有可能预测个体肿瘤的抗癌药物活性。
