Chaudhuri A G, Ganguly U
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
Biochim Biophys Acta. 1995 Jun 20;1267(2-3):131-3. doi: 10.1016/0167-4889(95)00055-w.
In Escherichia coli heat stable enterotoxin (STa)-treated rat enterocytes, the rise of inositol triphosphate (IP3) preceded the rise of [Ca2+]i. Chelation of extracellular Ca2+ with EGTA and suspension of cells in Ca2+ free buffer both demonstrated the enterotoxin-induced initial rise of [Ca2+]i with a concomitant loss of sustained phase. Furthermore, pretreatment of cells with dantrolene resulted in a decrease of the early response of [Ca2+]i, indicating the initial effect of the rise of [Ca2+]i was mostly due to its mobilization from some IP3-sensitive intracellular stores.
