Izumi S, Manabe A, Tomoyasu A, Kihara-Negishi F, Ariga H
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Biochim Biophys Acta. 1995 Jun 20;1267(2-3):135-8. doi: 10.1016/0167-4889(95)00071-y.
A cDNA encoding M2-type pyruvate kinase from mouse was cloned and its nucleotide sequence was determined. The cDNA encoded a protein containing 531 amino acids, the nucleotide and amino acid sequences of which were 93.2% and 98.1%, respectively, homologous to those of rat M2 pyruvate kinase. Expression of the pyruvate kinase in mouse embryonal carcinoma P19 cells altered according to differentiation stages; high at undifferentiated and low at differentiated stages.
从小鼠中克隆出编码M2型丙酮酸激酶的cDNA，并测定了其核苷酸序列。该cDNA编码一种含有531个氨基酸的蛋白质，其核苷酸序列和氨基酸序列分别与大鼠M2丙酮酸激酶的核苷酸序列和氨基酸序列具有93.2%和98.1%的同源性。丙酮酸激酶在小鼠胚胎癌P19细胞中的表达根据分化阶段而改变；在未分化阶段高，在分化阶段低。
