Márová I, Záhejský J, Sehnalová H
Department of Dermatology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Acta Diabetol. 1995 Mar;32(1):38-43. doi: 10.1007/BF00581043.
A selected group of diabetic patients showed a statistically significant increase in levels of glycated proteins in the stratum corneum compared with a control group. The values of glycated proteins correlated with those of glycohaemoglobin (GHb), and in diabetic patients also with serum glucose concentrations. The values of glycated proteins (and GHb) exhibited a positive correlation with age both in a control group and in diabetic patients. The average values of glycated proteins (and GHb) were slightly higher in women than in men. Determination of glycated proteins levels of the stratum corneum can serve as a stable parameter for long-term monitoring of the course of non-enzymatic glycation in structural and connective tissues and thus also for the prognosis of the development of dermatological complications related to diabetes mellitus. In vitro incubation of stratum corneum proteins and keratin with glucose resulted in an increase of their glycation. The values of glycated proteins and glycated keratin increased proportionally to the glucose concentration and duration of incubation. Glucose binding to keratin and proteins of the insoluble stratum corneum fraction appeared to occur at practically the same rate, and it is a first-order reaction with regard to the glucose concentration. Water-soluble proteins of the stratum corneum undergo non-enzymatic glycation preferentially (on average, 83.4% of the total amount of glycated proteins is present in the soluble fraction), regardless of the initial content of glycated proteins in the sample. The content of glycated soluble proteins of a higher molecular weight significantly increased after 4 weeks of incubation with glucose.
与对照组相比，一组选定的糖尿病患者角质层中糖化蛋白水平在统计学上有显著升高。糖化蛋白的值与糖化血红蛋白（GHb）的值相关，在糖尿病患者中还与血糖浓度相关。糖化蛋白（和GHb）的值在对照组和糖尿病患者中均与年龄呈正相关。糖化蛋白（和GHb）的平均值女性略高于男性。测定角质层糖化蛋白水平可作为长期监测结构和结缔组织中非酶糖基化进程的稳定参数，从而也可用于预测与糖尿病相关的皮肤并发症的发展。角质层蛋白和角蛋白与葡萄糖的体外孵育导致它们的糖基化增加。糖化蛋白和糖化角蛋白的值与葡萄糖浓度和孵育时间成正比增加。葡萄糖与不溶性角质层部分的角蛋白和蛋白质的结合似乎以几乎相同的速率发生，并且就葡萄糖浓度而言是一级反应。角质层的水溶性蛋白优先发生非酶糖基化（平均而言，糖化蛋白总量的83.4%存在于可溶部分），与样品中糖化蛋白的初始含量无关。与葡萄糖孵育4周后，较高分子量的糖化可溶性蛋白含量显著增加。
