Chano T, Ishizawa M, Matsumoto K, Morimoto S, Hukuda S, Okabe H
Department of Orthopaedic Surgery, Shiga University of Medical Science.
Eur J Histochem. 1995;39(1):21-30.
S-100 protein (S-100) appears to be a marker for bone tumors of cartilaginous origin. Any analyses of proliferative activity in S-100-positive tumor cells, however, has not yet been presented. This study assessed the proliferative activity of those cells by means of a double-immunohistochemical staining method using proliferating cell nuclear antigen (PCNA) and S-100. The most intense reactivity for S-100 was found in the well-differentiated chondrocytes of enchondromas, osteochondromas, and osteosarcomas. On the contrary, the more immature the tumor cells were, the more intensely positive they were for PCNA. In parosteal chondrosarcoma, exceptionally, PCNA-positive as well as S-100-positive cells were abundant, suggesting that these proliferating cells produced S-100. In periosteal osteosarcoma, however, the proliferating cells labeled by PCNA revealed little reactivity for S-100. This immunohistochemical method is potentially useful to know the identity and origin of proliferating cells and may sometimes be diagnostic for bone tumors containing cartilaginous elements.
S-100蛋白（S-100）似乎是软骨源性骨肿瘤的标志物。然而，尚未有关于S-100阳性肿瘤细胞增殖活性的分析报告。本研究采用增殖细胞核抗原（PCNA）和S-100的双重免疫组织化学染色方法评估这些细胞的增殖活性。在内生软骨瘤、骨软骨瘤和骨肉瘤的高分化软骨细胞中发现S-100的反应最强。相反，肿瘤细胞越不成熟，其PCNA阳性程度越高。在骨旁软骨肉瘤中，PCNA阳性和S-100阳性细胞都很丰富，这表明这些增殖细胞产生S-100。然而，在骨膜骨肉瘤中，PCNA标记的增殖细胞对S-100几乎没有反应。这种免疫组织化学方法可能有助于了解增殖细胞的特性和来源，有时对含有软骨成分的骨肿瘤具有诊断价值。
