Chano T, Matsumoto K, Ishizawa M, Morimoto S, Hukuda S, Okabe H
Department of Orthopedics, Shiga University of Medical Science, Japan.
Acta Orthop Scand. 1994 Jun;65(3):355-8. doi: 10.3109/17453679408995471.
Differentiation of periosteal osteosarcoma and parosteal (periosteal) chondrosarcoma by conventional histology may be difficult. One case each of clinically and histologically proven periosteal osteosarcoma and parosteal chondrosarcoma were evaluated by a double-immunohistochemical staining method using proliferating cell nuclear antigen (PCNA) and S-100 protein (S-100). Conventional histology showed proliferation of both osteoblastic and chondroblastic cells in the periosteal osteosarcoma, while there was a growth of only chondroblastic tumor cells in the parosteal chondrosarcoma. Immunohistochemical studies indicated that the nuclei of chondroblastic cells recognized by S-100 were PCNA-negative, while osteoblastic stromal cells were PCNA-positive in the periosteal osteosarcoma. In contrast, chondroblastic cells in the parosteal chondrosarcoma were both S-100- and PCNA-positive. Our findings suggest that periosteal osteosarcoma is characterized by the proliferation of osteoblastic stromal cells, whereas parosteal chondrosarcoma is characterized by the proliferation of chondroblastic cells. This method of double immunohistochemical staining, using PCNA and S-100, may be useful in differentiating these chondroblastic tumors.
通过传统组织学方法鉴别骨膜骨肉瘤和骨旁（骨膜）软骨肉瘤可能存在困难。采用增殖细胞核抗原（PCNA）和S-100蛋白（S-100）双重免疫组织化学染色法对1例经临床和组织学证实的骨膜骨肉瘤及1例骨旁软骨肉瘤进行评估。传统组织学显示，骨膜骨肉瘤中同时存在成骨细胞和软骨母细胞增殖，而骨旁软骨肉瘤中仅见软骨母细胞瘤细胞生长。免疫组织化学研究表明，在骨膜骨肉瘤中，S-100识别的软骨母细胞核PCNA阴性，而成骨基质细胞PCNA阳性。相反，骨旁软骨肉瘤中的软骨母细胞S-100和PCNA均为阳性。我们的研究结果提示，骨膜骨肉瘤以成骨基质细胞增殖为特征，而骨旁软骨肉瘤以软骨母细胞增殖为特征。这种使用PCNA和S-100的双重免疫组织化学染色方法可能有助于鉴别这些软骨母细胞瘤。
