Chano T, Matsumoto K, Ishizawa M, Morimoto S, Hukuda S, Okabe H, Kato H, Fujino S
Department of Orthopaedic Surgery, Shiga University of Medical Science, Japan.
Eur J Histochem. 1996;40(3):189-98.
Osteosarcomas are characterized by different histologic subtypes that are composed of heterogeneous tumor cells. Although the histological origin of the malignant cells is unknown, it has been speculated that osteoblasts lead to the malignant cells. In the current study, the osteosarcoma cells in 27 lesions were assessed by means of immunohistochemical staining for osteocalcin (OC), S-100 protein (S-100) and proliferating cell nuclear antigen (PCNA). PCNA labeling indices were the highest in osteoblastic and stromal areas, and significantly lower in chondroblastic areas (p < 0.01). Cells that were positive for both PCNA and OC were abundant in osteoblastic and stromal areas, while cells that were positive for both PCNA and S-100 were rarely observed. These results were almost similar for conventional, parosteal and periosteal osteosarcomas. In contrast, OC reactivity was poor in fibroblastic osteosarcoma, in osteosarcoma with giant cells, and in telangiectatic osteosarcoma. Pulmonary metastatic osteosarcoma lesions weakly expressed OC (p < 0.01), but showed high values for the PCNA labeling indices. In conclusion, immunohistochemical staining for OC, S-100, and PCNA are useful to analyze the proliferating cells in osteosarcomas. The main proliferating cells in most osteosarcomas are mature osteoblast-like cells. OC-negative tumor cells predominate in some of osteosarcoma subtypes, and these tumors therefore probably represent a distinct osteosarcoma variant. OC expression in pulmonary metastatic lesions may be suppressed.
骨肉瘤具有不同的组织学亚型，由异质性肿瘤细胞组成。尽管恶性细胞的组织学起源尚不清楚，但据推测成骨细胞会导致恶性细胞的产生。在本研究中，通过对骨钙素（OC）、S-100蛋白（S-100）和增殖细胞核抗原（PCNA）进行免疫组织化学染色，对27个病灶中的骨肉瘤细胞进行了评估。PCNA标记指数在成骨和基质区域最高，而在软骨母细胞区域显著较低（p < 0.01）。PCNA和OC均呈阳性的细胞在成骨和基质区域丰富，而PCNA和S-100均呈阳性的细胞很少观察到。对于传统型、骨旁型和骨膜型骨肉瘤，这些结果几乎相似。相比之下，在纤维母细胞型骨肉瘤、伴有巨细胞的骨肉瘤和毛细血管扩张型骨肉瘤中，OC反应性较差。肺转移骨肉瘤病灶中OC表达较弱（p < 0.01），但PCNA标记指数较高。总之，对OC、S-100和PCNA进行免疫组织化学染色有助于分析骨肉瘤中的增殖细胞。大多数骨肉瘤中的主要增殖细胞是成熟的成骨样细胞。OC阴性肿瘤细胞在某些骨肉瘤亚型中占主导地位，因此这些肿瘤可能代表一种独特的骨肉瘤变体。肺转移病灶中OC的表达可能受到抑制。
