环状芽孢杆菌β-半乳糖苷酶催化乳糖形成寡糖。
Formation of oligosaccharides from lactose by Bacillus circulans beta-galactosidase.
作者信息
Yanahira S, Kobayashi T, Suguri T, Nakakoshi M, Miura S, Ishikawa H, Nakajima I
机构信息
Technical Research Institute, Snow Brand Milk Products Co., Ltd., Saitama, Japan.
出版信息
Biosci Biotechnol Biochem. 1995 Jun;59(6):1021-6. doi: 10.1271/bbb.59.1021.
Eleven oligosaccharides formed by a transglycosylation reaction during lactose hydrolysis with Bacillus circulans beta-galactosidase were purified by gel permeation chromatography, charcoal chromatography, and HPLC. From the results of methylation analysis, and MS and NMR studies, it was concluded that these oligosaccharides were beta-D-Galp-(1-->3)-D-Glc, beta-D-Galp-(1-->6)-D-Glc, beta-D-Galp-(1-->2)-D-Glc, beta-D-Galp-(1-->4)-beta-D-Galp-(1-->4)-D-Glc, beta-D-Galp-(1-->6)-[beta-D-Galp-(1-->2)]-D-Glc, beta-D-Galp-(1-->6)-[beta-D-Galp-(1-->4)]-D-Glc, beta-D-Galp-(1-->4)-beta-D-Galp-(1-->3)-D-Glc, beta-D-Galp-(1-->4)-beta-D-Galp-(1-->2)-D-Glc, beta-D-Galp-(1-->4)-[beta-D-Galp-(1-->2)]-D-Glc, beta-D-Galp-(1-->4)-beta-D-Galp-(1-->6)-D-Glc, beta-D-Galp-(1-->6)[beta-D-Galp-(1-->3)]-D-Glc. The last five are newly observed oligosaccharides. The results of a use test (in vitro) by human intestinal bacteria showed that the oligosaccharides containing lactose units were predominantly used by human intestinal bifidobacteria.
利用环状芽孢杆菌β-半乳糖苷酶进行乳糖水解过程中通过转糖基化反应形成的11种低聚糖，通过凝胶渗透色谱、活性炭色谱和高效液相色谱进行了纯化。根据甲基化分析、质谱和核磁共振研究结果，得出这些低聚糖为β-D-吡喃半乳糖基-(1→3)-D-葡萄糖、β-D-吡喃半乳糖基-(1→6)-D-葡萄糖、β-D-吡喃半乳糖基-(1→2)-D-葡萄糖、β-D-吡喃半乳糖基-(1→4)-β-D-吡喃半乳糖基-(1→4)-D-葡萄糖、β-D-吡喃半乳糖基-(1→6)-[β-D-吡喃半乳糖基-(1→2)]-D-葡萄糖、β-D-吡喃半乳糖基-(1→6)-[β-D-吡喃半乳糖基-(1→4)]-D-葡萄糖、β-D-吡喃半乳糖基-(1→4)-β-D-吡喃半乳糖基-(1→3)-D-葡萄糖、β-D-吡喃半乳糖基-(1→4)-β-D-吡喃半乳糖基-(1→2)-D-葡萄糖、β-D-吡喃半乳糖基-(1→4)-[β-D-吡喃半乳糖基-(1→2)]-D-葡萄糖、β-D-吡喃半乳糖基-(1→4)-β-D-吡喃半乳糖基-(1→6)-D-葡萄糖、β-D-吡喃半乳糖基-(1→6)[β-D-吡喃半乳糖基-(1→3)]-D-葡萄糖。最后五种是新发现的低聚糖。人体肠道细菌的使用测试（体外）结果表明，含乳糖单元的低聚糖主要被人体肠道双歧杆菌利用。
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