Mori A, Suko M, Tsuruoka N, Kaminuma O, Ohmura T, Nishizaki Y, Ito K, Okudaira H
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan.
Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):220-2. doi: 10.1159/000236983.
CD4+ T cell clones specific for Der fII (a major allergen of the house dust mite) were established from peripheral blood mononuclear cells of atopic patients. All of the T cell clones were classified as having the Th0 phenotype, since they produced both interleukin (IL)-2 and IL-4 upon stimulation. Some of the clones produced IL-5 upon antigenic stimulation. Human recombinant IL-2 induced these T cell clones to express IL-5 mRNA and produce IL-5 protein in a dose-dependent manner. IL-2 did not induce IL-4 production, indicating a discrete signal requirement for IL-4 versus IL-5 production by T cells. Moreover, IL-5 production induced by immobilized anti-CD3 monoclonal antibody was completely suppressed by the addition of anti-IL-2 monoclonal antibody, suggesting that IL-5 production, designated as a Th2-type immune response, is dependent on IL-2, a Thl cytokine. IL-2 produced at the site of allergic inflammation may contribute to IL-5 production by T cells in vivo.
从特应性患者的外周血单核细胞中建立了对Der fII（屋尘螨的主要变应原）特异的CD4 + T细胞克隆。所有T细胞克隆均被归类为具有Th0表型，因为它们在受到刺激后会产生白细胞介素（IL）-2和IL-4。一些克隆在抗原刺激下会产生IL-5。人重组IL-2以剂量依赖的方式诱导这些T细胞克隆表达IL-5 mRNA并产生IL-5蛋白。IL-2不诱导IL-4产生，表明T细胞产生IL-4和IL-5需要不同的信号。此外，固定化抗CD3单克隆抗体诱导的IL-5产生被抗IL-2单克隆抗体的加入完全抑制，这表明被指定为Th2型免疫反应的IL-5产生依赖于Th1细胞因子IL-2。在过敏性炎症部位产生的IL-2可能有助于体内T细胞产生IL-5。
