Gutgesell C, Yssel H, Scheel D, Gerdes J, Neumann C
Department of Dermatology, University of Göttingen, Germany.
Exp Dermatol. 1994 Dec;3(6):304-13. doi: 10.1111/j.1600-0625.1994.tb00293.x.
In atopic individuals, allergen-specific CD4+ T lymphocytes often belong to the T-helper 2 (Th2) subset as they secrete the marker cytokines interleukin-4 (IL-4) and IL-5 but not interferon-gamma (INF-gamma). IL-10 is a cytokine the production of which, in the mouse system has been described to be restricted to the Th2 subset, but in the human was found to be produced by both Th1 and Th2 T cell clones (TCC). We have recently shown that house dust mite antigen (Dermatophagoides pteronyssinus)-specific TCC isolated from skin of patients with atopic dermatitis have a more polarized Th2 cytokine production profile than TCC obtained from the peripheral blood of these patients. In this study, we report that skin-derived TCC secrete more IL-10, IL-4 and IL-5, than TCC isolated from the blood of the same individual (p < 0.05). The difference was more significant with specific TCC than with non-specific TCC. Furthermore, there was a positive correlation between the production of IL-10 and that of IL-4 and IL-5, respectively. In addition, the amount of IL-4 and IL-5 secreted by specific TCC from the skin correlated positively. These results were confirmed by the detection of mRNA by PCR. Finally, our data confirm that in human blood-derived TCC IL-10 secretion is not related to a particular cytokine production profile. We suggest that the skin of AD provides an unique environment for the development of aTh2-like secretion pattern not only with respect to IL-4 and IL-5 but also regarding IL-10.
在特应性个体中，变应原特异性CD4+ T淋巴细胞通常属于辅助性T细胞2（Th2）亚群，因为它们分泌标志性细胞因子白细胞介素-4（IL-4）和IL-5，但不分泌干扰素-γ（INF-γ）。IL-10是一种细胞因子，在小鼠系统中其产生被认为仅限于Th2亚群，但在人类中发现Th1和Th2 T细胞克隆（TCC）均可产生。我们最近发现，从特应性皮炎患者皮肤中分离出的屋尘螨抗原（粉尘螨）特异性TCC，比从这些患者外周血中获得的TCC具有更极化的Th2细胞因子产生谱。在本研究中，我们报告皮肤来源的TCC比从同一个体血液中分离出的TCC分泌更多的IL-10、IL-4和IL-5（p < 0.05）。特异性TCC的差异比非特异性TCC更显著。此外，IL-10的产生与IL-4和IL-5的产生之间分别存在正相关。另外，皮肤特异性TCC分泌的IL-4和IL-5量也呈正相关。这些结果通过PCR检测mRNA得到证实。最后，我们的数据证实，在人血源性TCC中，IL-10的分泌与特定的细胞因子产生谱无关。我们认为，特应性皮炎患者的皮肤不仅在IL-4和IL-5方面，而且在IL-10方面，为类似Th2分泌模式的发展提供了独特的环境。
