Bitoh S, Wakefield R, Lang G M, Sehon A H
Immunology Department, University of Manitoba, Winnipeg, Canada.
Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):316-8. doi: 10.1159/000237012.
In this study we established that the conjugate of ovalbumin (OVA), which was used as a model allergen, with monomethoxypolyethylene glycol (mPEG), i.e., OVA(mPEG)11, was not only tolerogenic and essentially non-allergenic, but also capable of inactivating mast cells sensitized with anti-OVA IgE antibodies (Abs). Moreover, mast cells sensitized with a mixture of anti-OVA and anti-DNP IgE Abs were also desensitized by OVA(mPEG)11 with respect to both sensitivities. Most importantly, treatment of OVA-sensitive mice by OVA(mPEG)11 protected them from systemic anaphylaxis on challenge with OVA. The possibility of inactivating IgE-sensitized mast cells with mPEG conjugates of single epitopes of a given allergen is being investigated.
在本研究中，我们证实，作为模型变应原的卵清蛋白（OVA）与单甲氧基聚乙二醇（mPEG）的缀合物，即OVA(mPEG)11，不仅具有耐受性且基本无变应原性，还能够使被抗OVA IgE抗体（Abs）致敏的肥大细胞失活。此外，用抗OVA和抗DNP IgE Abs混合物致敏的肥大细胞在两种敏感性方面也被OVA(mPEG)11脱敏。最重要的是，用OVA(mPEG)11处理OVA敏感小鼠可使其在受到OVA攻击时免受全身性过敏反应。目前正在研究用给定变应原单个表位的mPEG缀合物使IgE致敏肥大细胞失活的可能性。
