Wyse B W, Wittwer C, Hansen R G
Clin Chem. 1979 Jan;25(1):108-10.
We describe a radioimmunoassay for pantothenic acid in biological tissues. D-Pantothenic acid was conjugated with bovine serum albumin by use of a bromoacetyl derivative of pantothenic acid, and antibody to this antigen was raised by injecting it into the foot pads of rabbits. For the radioimmunoassay, a 100-fold dilution of the resulting antiserum was incubated with radiolabeled pantothentic acid. The antibodies were precipitated and dissolved, and the radioactivity of the solution was measured in a liquid scintillation counter. Between 5 and 125 ng of pantothenic acid can be detected in 75 muL of tissue extract. Validation included recovery and precision studies, parallelism with tissue extracts, and competitive binding studies. Results of the radioimmunoassay and those of microbiological assay with use of Lactobacillus plantarum correlated well (r = 0.80).
我们描述了一种用于生物组织中泛酸的放射免疫测定法。利用泛酸的溴乙酰衍生物将D-泛酸与牛血清白蛋白偶联，并将其注射到兔足垫中以产生针对该抗原的抗体。在放射免疫测定中，将所得抗血清稀释100倍后与放射性标记的泛酸一起孵育。抗体沉淀并溶解后，在液体闪烁计数器中测量溶液的放射性。在75μL组织提取物中可检测到5至125 ng泛酸。验证包括回收率和精密度研究、与组织提取物的平行性以及竞争性结合研究。放射免疫测定结果与使用植物乳杆菌的微生物测定结果相关性良好（r = 0.80）。
