杜邦aca氨法的性能

Performance of the Du Pont aca ammonia method.

作者信息

Doumas B T, Hause L L, Sciacca R D, Jendrzejczak B, Foreback C C, Hoover J D, Spencer W W, Smock P L

出版信息

Clin Chem. 1979 Jan;25(1):175-8.

Abstract

We evaluated the performance of the Du Pont aca ammonia procedure with regard to (a) linearity, (b) precision, (c) interferences, and (d) effect of anticoagulants. Linearity extends to 2,000 mumol/L. The SD of the method was essentially constant (2 to 3 mumol/L) and independent of the NH3 concentration. Hemoglobin, bilirubin, and lipemia do not interfere. Either EDTA or heparin is suitable as anticoagulant. Recovery of NH3 added to plasma samples averaged 102% (range: 97--107%). We established normal values by measuring NH3 in 188 plasma samples from apparently healthy individuals. The 95% confidence range is from 10 to 35 mumol/L. The aca ammonia method compares very well with that of Kingsley and Tager but correlates less strongly with that of Reinhold and Chung. We describe a protein-based solution with stable NH3 concentration that is suitable as a control material.

摘要

我们评估了杜邦aca氨测定法在以下方面的性能：（a）线性，（b）精密度，（c）干扰因素，以及（d）抗凝剂的影响。线性范围可达2000μmol/L。该方法的标准差基本恒定（2至3μmol/L），且与氨浓度无关。血红蛋白、胆红素和脂血均无干扰。乙二胺四乙酸（EDTA）或肝素均可作为合适的抗凝剂。添加到血浆样本中的氨的回收率平均为102%（范围：97%至107%）。我们通过测量188名明显健康个体的血浆样本中的氨来确定正常值。95%置信区间为10至35μmol/L。aca氨测定法与金斯利和塔杰的方法相比非常好，但与莱因霍尔德和钟的方法相关性较弱。我们描述了一种氨浓度稳定的基于蛋白质的溶液，适合作为对照物质。