Doumas B T, Hause L L, Sciacca R D, Jendrzejczak B, Foreback C C, Hoover J D, Spencer W W, Smock P L
Clin Chem. 1979 Jan;25(1):175-8.
We evaluated the performance of the Du Pont aca ammonia procedure with regard to (a) linearity, (b) precision, (c) interferences, and (d) effect of anticoagulants. Linearity extends to 2,000 mumol/L. The SD of the method was essentially constant (2 to 3 mumol/L) and independent of the NH3 concentration. Hemoglobin, bilirubin, and lipemia do not interfere. Either EDTA or heparin is suitable as anticoagulant. Recovery of NH3 added to plasma samples averaged 102% (range: 97--107%). We established normal values by measuring NH3 in 188 plasma samples from apparently healthy individuals. The 95% confidence range is from 10 to 35 mumol/L. The aca ammonia method compares very well with that of Kingsley and Tager but correlates less strongly with that of Reinhold and Chung. We describe a protein-based solution with stable NH3 concentration that is suitable as a control material.
我们评估了杜邦aca氨测定法在以下方面的性能：（a）线性，（b）精密度，（c）干扰因素，以及（d）抗凝剂的影响。线性范围可达2000μmol/L。该方法的标准差基本恒定（2至3μmol/L），且与氨浓度无关。血红蛋白、胆红素和脂血均无干扰。乙二胺四乙酸（EDTA）或肝素均可作为合适的抗凝剂。添加到血浆样本中的氨的回收率平均为102%（范围：97%至107%）。我们通过测量188名明显健康个体的血浆样本中的氨来确定正常值。95%置信区间为10至35μmol/L。aca氨测定法与金斯利和塔杰的方法相比非常好，但与莱因霍尔德和钟的方法相关性较弱。我们描述了一种氨浓度稳定的基于蛋白质的溶液，适合作为对照物质。
