Jawad M J, Wilson E A, Kincaid H L
Clin Chem. 1979 Jan;25(1):99-102.
We report a rapid double-antibody radioimmunoassay for urinary estriol. Advantages over other current methods include: (a) 30-min hydrolysis; (b) total incubation time, 55 min; (c) assay unaffected by urinary glucose; (d) no degradation of estriol evident during hydrolysis; (e) superior (85%) analytical recovery of estriol conjugates; (f) linear standard curve by logit-log extrapolation; (g) good correlation (r = 0.83) with total estrogen determination by a generally accepted colorimetric method; (h) only 20 muL of urine required; and (i) the detection range is 1.9 to 100.5 mg/24-h urine.
我们报告了一种用于检测尿雌三醇的快速双抗体放射免疫测定法。与目前其他方法相比，其优点包括：(a) 30分钟水解；(b) 总孵育时间55分钟；(c) 测定不受尿糖影响；(d) 水解过程中雌三醇无明显降解；(e) 雌三醇结合物的分析回收率高（85%）；(f) 通过对数-对数外推法得到线性标准曲线；(g) 与普遍接受的比色法测定总雌激素有良好的相关性（r = 0.83）；(h) 仅需20微升尿液；(i) 检测范围为1.9至100.5毫克/24小时尿液。
