Anderson D W, Goebelsmann U
Clin Chem. 1976 May;22(5):611-5.
We describe a radioimmunoassay for total urinary estriol in pregnancy. A 25-mul aliquot of the urine specimen is acid hydrolyzed, neutralized, and diluted before assay. We use rabbit antisera against estriol-6-(O-carboxymethyl)oxime/bovine serum albumin and ammonium sulfate precipitation at room temperature. Results are unaffected by glucose or methenamine mandelate, a urinary tract antiseptic. Using semi-automatic pipetting equipment, one laboratory technologist can complete 50 assays within 8 h. This technique is both reliable and convenient and should decrease the expense of routine estriol assays.
我们描述了一种用于检测孕期尿中总雌三醇的放射免疫分析法。尿标本取25微升等分试样，在检测前进行酸水解、中和及稀释。我们使用针对雌三醇-6-(O-羧甲基)肟/牛血清白蛋白的兔抗血清，并在室温下用硫酸铵沉淀。结果不受葡萄糖或尿路防腐剂乌洛托品扁桃酸盐的影响。使用半自动移液设备，一名实验室技术人员在8小时内可完成50次检测。该技术既可靠又方便，且应能降低常规雌三醇检测的费用。
