Van der Laarse A, Graf-Minor M L, Witteveen S A
Clin Chim Acta. 1979 Jan 1;91(1):47-52. doi: 10.1016/0009-8981(79)90469-8.
In a monolayer cultures of neonatal rat heart cells deprived of oxygen and metabolic substrates we measured (1) the release of hypoxanthine, a product of ATP catabolism, and (2) enzyme depletion with creatine phosphokinase (CPK) and alpha-hydroxybutyrate dehydrogenase (alpha-HBDH). Taking samples each hour up to 7 h of anoxia we were able to demonstrate that hypoxanthine release precedes cellular enzyme depletion. The release rate of hypoxanthine is maximal in the second hour of anoxia and the depletion rate of CPK and alpha-HBDH is maximal in the fourth hour. These results suggest that for early diagnosis of damage to heart cells due to deprivation of oxygen and metabolic substrates the measurement of hypoxanthine release is preferred to that of enzyme release.
在新生大鼠心脏细胞的单层培养物中，去除氧气和代谢底物后，我们测量了：（1）次黄嘌呤的释放，它是ATP分解代谢的产物；（2）肌酸磷酸激酶（CPK）和α-羟丁酸脱氢酶（α-HBDH）的酶耗竭情况。在缺氧长达7小时的过程中，每小时取样一次，我们能够证明次黄嘌呤的释放在细胞酶耗竭之前。次黄嘌呤的释放速率在缺氧的第二个小时达到最大值，而CPK和α-HBDH的耗竭速率在第四个小时达到最大值。这些结果表明，对于因缺氧和代谢底物缺乏导致的心脏细胞损伤的早期诊断，测量次黄嘌呤的释放比测量酶的释放更可取。
