Bruynzeel P L, Houben L A, Berrens L
Clin Chim Acta. 1979 Jan 1;91(1):67-74. doi: 10.1016/0009-8981(79)90472-8.
The paper radioimmunosorbent test (PRIST) has been described as the method of choice for determining low serum IgE levels; the radioimmunosorbent test (RIST) as the method of choice for determining normal and elevated serum IgE levels. By replacing the 125I-labelled anti-IgE antibody used in the paper radioimmunosorbent test by the 125I-labelled anti-IgE reagent used in radioallergosorbent test (RAST) and by changing the serum dilution and the incubation time, this modified sandwich technique (MST) became comparable to the RIST in the normal and elevated IgE-region and showed results similar to the PRIST in the very low IgE-region. The affinity of the 125I-labelled anti-IgE of the RAST proved to be about 2.6 times higher than the antibody used in the PRIST, which explains the improved results in the normal and the good results in the very low IgE-region. The lowest serum IgE level measurable by this method was as low as 0.05 I.U./ml, as determined in 20 cord sera. The mean IgE level in cord sera was 0.45 I.U./ml (range 0.05--2.63 I.U./ml). The results of this study suggest replacement of the antiserum used in PRIST by the one available for RAST.
纸上放射免疫吸附试验（PRIST）被认为是测定低血清IgE水平的首选方法；放射免疫吸附试验（RIST）则是测定正常和升高血清IgE水平的首选方法。通过用放射变应原吸附试验（RAST）中使用的125I标记抗IgE试剂替代纸上放射免疫吸附试验中使用的125I标记抗IgE抗体，并改变血清稀释度和孵育时间，这种改良的夹心技术（MST）在正常和升高的IgE区域与RIST相当，在极低IgE区域显示出与PRIST相似的结果。事实证明，RAST的125I标记抗IgE的亲和力比PRIST中使用的抗体高约2.6倍，这解释了在正常区域结果的改善以及在极低IgE区域的良好结果。通过该方法可测量的最低血清IgE水平低至0.05 I.U./ml，这是在20份脐血血清中测定的。脐血血清中的平均IgE水平为0.45 I.U./ml（范围为0.05 - 2.63 I.U./ml）。本研究结果表明，可用RAST的抗血清替代PRIST中使用的抗血清。
