采用气相色谱-质谱联用技术鉴定生物材料中的4-N-2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖基-L-天冬酰胺
Identification of 4-N-2-acetamido-2-deoxy-beta-D-glucopyranosyl-L-asparagine in biological materials by gas chromatography-mass spectrometry.
作者信息
Maury P, Kärkkäinen J
出版信息
Clin Chim Acta. 1979 Jan 1;91(1):75-9. doi: 10.1016/0009-8981(79)90473-x.
A specific and sensitive method for the identification of 4-N-2-acetamido-2-deoxy-beta-D-glucopyranosyl-L-asparagine (GlcNAc-Asn) in urine in aspartylglycosaminuria and in hydrolysates of glycoproteins is described. The method involves permethylation of GlcNAc-Asn followed by gas chromatographic-mass spectrometric analysis of the methylated derivative. It can be used to confirm the diagnosis of aspartylglycosaminuria and to assess the excretion of GlcNAc-Asn in urine during various phases of the disease. The presence of an N-acetylglucosaminyl-asparagine type of carbohydrate-peptide linkage in a glycoprotein can be determined by applying the method to the partial acid hydrolysate of a proteolytically digested glycoprotein.
描述了一种用于鉴定天冬氨酰葡糖胺尿症患者尿液以及糖蛋白水解产物中4-N-2-乙酰氨基-2-脱氧-β-D-吡喃葡糖基-L-天冬酰胺（GlcNAc-Asn）的特异性和灵敏性方法。该方法包括对GlcNAc-Asn进行全甲基化，然后对甲基化衍生物进行气相色谱-质谱分析。它可用于确诊天冬氨酰葡糖胺尿症，并评估疾病各个阶段尿液中GlcNAc-Asn的排泄情况。通过将该方法应用于经蛋白酶消化的糖蛋白的部分酸水解产物，可以确定糖蛋白中是否存在N-乙酰葡糖胺基-天冬酰胺类型的碳水化合物-肽键。
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