Sharrocks A D
Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, UK.
FEBS Lett. 1995 Jul 10;368(1):77-80. doi: 10.1016/0014-5793(95)00604-8.
A ternary complex comprised of SRF, ternary complex factor (TCF) and the c-fos SRE is the target of several extracellular signal regulated pathways. Phosphorylation of the TCF Elk-1 is a key event in the activation of this complex. We demonstrate that ERK2/p42 phosphorylation of Elk-1 stimulates its recruitment into ternary complexes with SRF. Moreover, phosphorylation of Elk-1 also stimulates its autonomous SRF-independent binding to high affinity binding sites. Thus part of the effect of ERK2/p42 phosphorylation is to stimulate DNA-binding by the ETS DNA-binding domain of Elk-1.
由血清反应因子（SRF）、三元复合因子（TCF）和c-fos血清反应元件（SRE）组成的三元复合物是几种细胞外信号调节途径的作用靶点。TCF Elk-1的磷酸化是该复合物激活过程中的关键事件。我们证明，ERK2/p42对Elk-1的磷酸化促进其与SRF形成三元复合物。此外，Elk-1的磷酸化还促进其在不依赖SRF的情况下自主结合高亲和力结合位点。因此，ERK2/p42磷酸化的部分作用是通过Elk-1的ETS DNA结合结构域刺激DNA结合。
