Styrna J
Department of Genetics and Evolution, Jagiellonian University, Kraków, Poland.
Genet Res. 1995 Jun;65(3):229-31. doi: 10.1017/s0016672300033309.
Cumulus-oocyte complexes from the two consomic strains of mice, B10.BR and B10.BR-Ydel (with a partial deletion of the Y chromosome) and the BALB/c strain, as well as reciprocal F1 and F2 hybrids, were tested for their susceptibility to attack by hyaluronidase. The cumulus cell dispersal was significantly more rapid in the eggs of B10.BR females and of F1 and F2 hybrid females sired by the B10.BR males than in those of BALB/c females and hybrids sired by BALB/c males. These results confirm the earlier data of Bander et al. (1989) which were interpreted as evidence of paternal imprinting of the C57BL genome. In contrast, cumulus cell dispersal in the eggs of females sired by B10.BR-Ydel males was significantly slower than in females sired by B10.BR males, and did not differ from that in the BALB/c strain. The results suggest that the partial deletion of the Y chromosome abolished the effect of paternal genome imprinting which is observed in the B10.BR strain.
对来自两种同源导入近交系小鼠（B10.BR和B10.BR - Ydel，后者Y染色体有部分缺失）、BALB/c品系以及正反交F1和F2杂种的卵丘 - 卵母细胞复合体进行了透明质酸酶攻击敏感性测试。B10.BR雌性小鼠以及由B10.BR雄性小鼠所生的F1和F2杂种雌性小鼠的卵中，卵丘细胞的分散速度明显快于BALB/c雌性小鼠以及由BALB/c雄性小鼠所生杂种的卵。这些结果证实了Bander等人（1989年）早期的数据，这些数据被解释为C57BL基因组父本印记的证据。相比之下，由B10.BR - Ydel雄性小鼠所生雌性小鼠的卵中卵丘细胞的分散速度明显慢于由B10.BR雄性小鼠所生的雌性小鼠，且与BALB/c品系无差异。结果表明，Y染色体的部分缺失消除了在B10.BR品系中观察到的父本基因组印记效应。
