Dishman R K, Warren J M, Youngstedt S D, Yoo H, Bunnell B N, Mougey E H, Meyerhoff J L, Jaso-Friedmann L, Evans D L
Department of Exercise Science, University of Georgia, Athens 30602-3654, USA.
J Appl Physiol (1985). 1995 Apr;78(4):1547-54. doi: 10.1152/jappl.1995.78.4.1547.
We studied whether voluntary running in an activity wheel moderates splenic natural killer (NK) cell cytotoxicity after footshock. Young (50-day) male Fischer 344 rats were randomly assigned to 1) sedentary (n = 16) or 2) activity-wheel (n = 16) groups that each received controllable or uncontrollable footshock on 2 consecutive days or 3) a sedentary home-cage control group (n = 8). Spleens and trunk blood were collected 30 min after the second footshock session. Cytotoxicity was determined by a standard 4-h 51Cr release assay. Percentages of OX6+ (B), OX8+ [T suppressor/cytotoxic (Ts/c)], W3/25+ (T helper), Thy-1.1 (Pan T cell marker), and 5C6+ (NK) cells were determined by flow cytometry. Plasma adrenocorticotropic hormone, corticosterone, and prolactin concentrations were measured by radioimmunoassay as modulators of NK activity. Percentage of specific lysis after footshock was approximately 52% of control values for sedentary animals compared with approximately 96% of control values for activity-wheel animals. The groups did not differ in percentages of NK or Ts/c cells. We conclude that voluntary activity-wheel running protects against the suppression of splenic NK activity induced by footshock. This protective effect of wheel running is not explained by an elevation in baseline NK activity; increased percentages of splenic NK or Ts/c cells; or plasma levels of adrenocorticotropic hormone, corticosterone, and prolactin.
我们研究了在遭受足部电击后，在活动轮中自愿跑步是否会调节脾脏自然杀伤（NK）细胞的细胞毒性。将年轻（50日龄）雄性Fischer 344大鼠随机分为1）久坐组（n = 16）或2）活动轮组（n = 16），每组连续2天接受可控或不可控的足部电击，或3）久坐笼养对照组（n = 8）。在第二次足部电击 session 后30分钟收集脾脏和躯干血液。通过标准的4小时51Cr释放试验测定细胞毒性。通过流式细胞术测定OX6 +（B）、OX8 + [T抑制/细胞毒性（Ts/c）]、W3/25 +（T辅助）、Thy-1.1（全T细胞标志物）和5C6 +（NK）细胞的百分比。通过放射免疫测定法测量血浆促肾上腺皮质激素、皮质酮和催乳素浓度作为NK活性的调节剂。与活动轮组动物对照值的约96%相比，足部电击后久坐动物的特异性裂解百分比约为对照值的52%。各组NK或Ts/c细胞的百分比没有差异。我们得出结论，自愿在活动轮中跑步可防止足部电击诱导的脾脏NK活性受到抑制。这种跑步的保护作用不能用基线NK活性升高、脾脏NK或Ts/c细胞百分比增加或血浆促肾上腺皮质激素、皮质酮和催乳素水平升高来解释。
