White-Welkley J E, Warren G L, Bunnell B N, Mougey E H, Meyerhoff J L, Dishman R K
Department of Exercise Science, University of Georgia, Athens 30602, USA.
J Appl Physiol (1985). 1996 Mar;80(3):931-9. doi: 10.1152/jappl.1996.80.3.931.
We examined whether rats that were treadmill exercise trained (Tr) or chronically immobilized (CI) had similar responses by the hypothalamic-pituitary-adrenal (HPA) cortical axis to acute stress and whether the HPA responses interacted with the hypothalamic-pituitary-gonadal (HPG) axis. After 6 wk (1 h/day, 6 days/wk) of Tr or CI, plasma concentrations of adrenocorticotropic hormone ([ACTH]), [prolactin], and [corticosterone] were measured after familiar (treadmill running or immobilization) or novel (footshock) stress. Ovariectomized Sprague-Dawley females (n = 72) were implanted with capsules containing estradiol benzoate (E2) and randomly assigned in a 2-group (E2 vs. no E2) x 3 treatment (Tr vs. CI vs. sedentary) x 4 acute stressor [footshock vs. treadmill running (Run) vs. immobilization (Im) vs. no stress] x 3 recovery time (1 vs. 15 vs. 30 min) mixed-model analysis of variance. E2 capsules were removed from one-half of the animals 48 h before the first stressor session. After 10 min of acute stress, blood was drawn from a jugular catheter at 1, 15, and 30 min of recovery. [ACTH] and [prolactin] after footshock were higher in Tr rats with E2 compared with CI and sedentary rats without E2; recovery levels for sedentary animals were higher after Run compared with Im. The elevation in [corticosterone] from minute 1 to 15 of recovery was higher after the familiar Run and Im conditions. Our findings are consistent with an increased responsiveness of the HPA axis to novel footshock after treadmill exercise training that is additionally modulated by the HPG axis.
我们研究了经跑步机运动训练（Tr）或长期固定（CI）的大鼠，其下丘脑 - 垂体 - 肾上腺（HPA）皮质轴对急性应激的反应是否相似，以及HPA反应是否与下丘脑 - 垂体 - 性腺（HPG）轴相互作用。在进行6周（每天1小时，每周6天）的Tr或CI处理后，在熟悉（跑步机跑步或固定）或新异（足部电击）应激后，测量促肾上腺皮质激素（[ACTH]）、[催乳素]和[皮质酮]的血浆浓度。对去卵巢的Sprague - Dawley雌性大鼠（n = 72）植入含苯甲酸雌二醇（E2）的胶囊，并随机分配到2组（E2组与无E2组）×3处理（Tr组与CI组与久坐组）×4急性应激源[足部电击与跑步机跑步（Run）与固定（Im）与无应激]×3恢复时间（1分钟与15分钟与30分钟）的混合模型方差分析。在第一次应激实验前48小时，从一半动物身上取出E2胶囊。急性应激10分钟后，在恢复的1分钟、15分钟和30分钟时，从颈静脉导管取血。与无E2的CI和久坐大鼠相比，有E2的Tr大鼠在足部电击后的[ACTH]和[催乳素]更高；与Im相比，久坐动物在Run后的恢复水平更高。在熟悉的Run和Im条件后，从恢复第1分钟到15分钟[皮质酮]的升高更高。我们的研究结果表明，跑步机运动训练后HPA轴对新异足部电击的反应性增加，且这种反应性还受到HPG轴的调节。
