Pistoor F H, Kapsenberg M L, Bos J D, Meinardi M M, von Blomberg M E, Scheper R J
Department of Dermatology, University of Amsterdam, The Netherlands.
J Invest Dermatol. 1995 Jul;105(1):92-5. doi: 10.1111/1523-1747.ep12313366.
Twenty Ni-reactive T-lymphocyte clones were obtained from eight different donors and analyzed for their ability to cross-react with other metals. All Ni-reactive T-lymphocyte clones were CD4+CD8- and recognized Ni in association with either HLA-DR or -DQ molecules. Based on the periodic table of the elements, the metals Cr, Fe, Co, Cu, and Zn from the same horizontal row as Ni, and Pd and Pt from the same vertical row, were selected to study T-lymphocyte clone cross-reactivity. Distinct cross-reactivity patterns were found that could be divided into three major groups: Ni-reactive T-lymphocyte clones i) cross-reacting with Cu, ii) cross-reacting with Pd, or iii) without cross-reactivity. Major histocompatibility complex class II-restriction patterns of Cu- and Pd-induced proliferative responses did not differ from those for the Ni-induced responses. In vitro cross-reactivities with Cu and Pd may be favored by their bivalency and location next to Ni in the periodic table, and the similarity of these metals to Ni in binding to histidine residues of peptides in the pocket of major histocompatibility complex class II molecules. The present findings suggest that Cu and Pd hypersensitivities, which are occasionally observed in Ni-allergic patients, may be due to cross-reactivities at the T-cell clonal level rather than to concomitant sensitization.
从8位不同供体中获得了20个对镍有反应的T淋巴细胞克隆，并分析了它们与其他金属发生交叉反应的能力。所有对镍有反应的T淋巴细胞克隆均为CD4 + CD8 -，并识别与HLA - DR或 - DQ分子相关的镍。根据元素周期表，选择与镍在同一横行的金属铬、铁、钴、铜和锌，以及与镍在同一纵行的钯和铂，来研究T淋巴细胞克隆的交叉反应性。发现了不同的交叉反应模式，可分为三大类：对镍有反应的T淋巴细胞克隆（i）与铜发生交叉反应，（ii）与钯发生交叉反应，或（iii）无交叉反应。铜和钯诱导的增殖反应的主要组织相容性复合体II类限制模式与镍诱导的反应没有差异。在体外，与铜和钯的交叉反应可能因其二价性以及在元素周期表中与镍相邻的位置，以及这些金属在与主要组织相容性复合体II类分子口袋中的肽的组氨酸残基结合方面与镍的相似性而受到促进。目前的研究结果表明，在镍过敏患者中偶尔观察到的铜和钯超敏反应可能是由于T细胞克隆水平的交叉反应，而非伴随的致敏作用。
