Chambaut-Guérin A M, Martinez M C, Hamimi C, Gauthereau X, Nunez J
INSERM U282, Hôpital Henri Mondor, Créteil, France.
J Neurochem. 1995 Aug;65(2):537-44. doi: 10.1046/j.1471-4159.1995.65020537.x.
The human neuroblastoma cell line SKNBE can be differentiated either by serum removal or by adding to the culture medium different morphogens, for instance, retinoic acid (RA), cyclic AMP derivatives, and phorbol esters. Both the differentiated and undifferentiated cells express the two types of membrane tumor necrosis factor (TNF) receptors (TNFRs) of 55 and 75 kDa (p55 and p75 TNFR, respectively) and also their soluble forms. After RA addition the number of the surface TNFRs per cell is increased approximately twofold, but the kinetics of expression are different, depending on the receptor type. The level of the mRNAs of 2.4 and 4.2 kb, which, respectively, encode the p55 and p75 TNFRs, is also increased during the time course of differentiation, and the kinetics of their expression are biphasic. In contrast, the number of TNFRs and the level of their encoding mRNAs remain unchanged after exposure of the cells to both a phorbol and a cyclic AMP derivative.
人神经母细胞瘤细胞系SKNBE可以通过去除血清或向培养基中添加不同的形态发生素来诱导分化，例如视黄酸（RA）、环磷酸腺苷衍生物和佛波酯。分化和未分化的细胞均表达两种膜肿瘤坏死因子（TNF）受体，分别为55 kDa和75 kDa（分别为p55和p75 TNFR）及其可溶性形式。添加RA后，每个细胞表面TNFR的数量增加约两倍，但表达动力学因受体类型而异。分别编码p55和p75 TNFR的2.4 kb和4.2 kb mRNA水平在分化过程中也会增加，其表达动力学呈双相性。相反，细胞暴露于佛波酯和环磷酸腺苷衍生物后，TNFR的数量及其编码mRNA的水平保持不变。
