Cho S H, Im J G, Choi Y S, Son Y S, Chung M H
Department of Food Science and Nutrition, Hyosung Women's University, Kyungbuk, Korea.
J Nutr Sci Vitaminol (Tokyo). 1995 Feb;41(1):61-72. doi: 10.3177/jnsv.41.61.
This study was conducted to investigate the protective role of vitamin E against the formations of lipid peroxides in plasma and tissues and of 8-hydroxydeoxyguanosine (8-OHdG) in livers of rats fed fish oil. Six-week-old male Sprague-Dawley rats were divided into four groups and fed experimental diets for 8 weeks. Three fish oil (F) groups were fed a menhaden fish oil and soybean oil (9:1) mixture as 10% (wt/wt) of their diet. These three groups (F0, FI, and FII) were provided with < or = 3, 45, and 209 IU of vitamin E/kg diet, respectively. The SI group was fed soybean oil with < or = 45 IU of vitamin E/kg diet. The F0 group had the highest levels of thiobarbituric acid-reactive substances (TBARS) in plasma (per milligram lipid), and liver, lung, heart, and kidney. The FI group had higher levels of TBARS than the SI group in plasma and tissues except the lung. In liver, the TBARS levels of the FII group were also higher than those of SI group, but in other tissues, similar levels were observed in the FII and SI groups. Plasma level of vitamin E was lowest in F0 group and vitamin E levels were generally lower in F groups than in SI group. These levels were expressed as vitamin E per milliliter of plasma. However, plasma E levels were similar when expressed per milligram plasma lipid. The liver 8-OHdG concentration tended to decrease as dietary vitamin E increased in the F groups, but there was no difference in the level of 8-OHdG between the FI and SI groups. These results suggest that vitamin E should be supplemented in fish oil feeding to prevent the enhanced lipid peroxidation and the formation of 8-OHdG in the body.
本研究旨在探讨维生素E对鱼油喂养大鼠血浆和组织中脂质过氧化物形成以及肝脏中8-羟基脱氧鸟苷（8-OHdG）形成的保护作用。六周龄雄性Sprague-Dawley大鼠分为四组，喂养实验性日粮8周。三个鱼油（F）组喂食鲱鱼油和大豆油（9:1）混合物，占其日粮的10%（重量/重量）。这三组（F0、FI和FII）分别给予≤3、45和209 IU维生素E/千克日粮。SI组喂食含≤45 IU维生素E/千克日粮的大豆油。F0组血浆（每毫克脂质）、肝脏、肺、心脏和肾脏中的硫代巴比妥酸反应性物质（TBARS）水平最高。除肺外，FI组血浆和组织中的TBARS水平高于SI组。在肝脏中，FII组的TBARS水平也高于SI组，但在其他组织中，FII组和SI组的水平相似。F0组血浆维生素E水平最低，F组的维生素E水平总体上低于SI组。这些水平以每毫升血浆中的维生素E表示。然而，以每毫克血浆脂质表示时，血浆E水平相似。F组肝脏中8-OHdG浓度随日粮维生素E增加而呈下降趋势，但FI组和SI组之间8-OHdG水平无差异。这些结果表明，在鱼油喂养时应补充维生素E，以防止体内脂质过氧化增强和8-OHdG的形成。
