Camins A, Escubedo E, Camarasa J, Carretero J, Newman A H, Kammüller M E
Laboratory of Pharmacology and Pharmacognosy, Faculty Pharmacy, University of Barcelona, Spain.
J Pharmacol Exp Ther. 1995 Jul;274(1):242-8.
The effect of AHN 086 [1-(2-isothiocyanatoethyl)-7-chloro-1,3-dihydro-5-(4- chloro-phenyl)-2H-1,4-benzodiazepine-2-one hydrochloride], an irreversible ligand of the mitochondrial benzodiazepine receptor, was studied in the heat shock protein (HSP) expression and the activation of dog neutrophils. At micromolar concentrations, AHN 086 induced a dose-dependent increase in free radical production (309 +/- 13% at 1 x 10(-5) M). This compound also increased the phagocytic activity (73 +/- 3.5% at 5 x 10(-6) M). Immunohistochemical results showed that AHN 086 induced the expression of HSP in neutrophil membranes. At 1 x 10(-5) M, the percentage of positive cells was: 90.2 (27 kD), 90.6 (72 kD) and 92.8 (90 kD). This expression was accompanied by a decrease in the presence of nuclear and cytoplasmic HSP. Flow cytometry studies showed that this cell membrane expression was located on the neutrophil surface and could be a consequence of HSP mobilization from nucleus and cytoplasm.
研究了线粒体苯二氮䓬受体不可逆配体AHN 086[1-(2-异硫氰酸根合乙基)-7-氯-1,3-二氢-5-(4-氯苯基)-2H-1,4-苯并二氮杂䓬-2-酮盐酸盐]对犬中性粒细胞热休克蛋白(HSP)表达及活化的影响。在微摩尔浓度下，AHN 086诱导自由基生成呈剂量依赖性增加(1×10⁻⁵M时为309±13%)。该化合物还增强了吞噬活性(5×10⁻⁶M时为73±3.5%)。免疫组织化学结果显示，AHN 086诱导中性粒细胞膜中HSP的表达。在1×10⁻⁵M时，阳性细胞百分比分别为：90.2(27kD)、90.6(72kD)和92.8(90kD)。这种表达伴随着细胞核和细胞质中HSP含量的减少。流式细胞术研究表明，这种细胞膜表达位于中性粒细胞表面，可能是HSP从细胞核和细胞质中动员的结果。
