Boulton M I, Haley C S, Springbett A J, Webb R
Department of Physiology, University Medical School, Edinburgh, UK.
J Reprod Fertil. 1995 Mar;103(2):199-207. doi: 10.1530/jrf.0.1030199.
The aim of this study was to investigate the role of FSH in the control of ovulation rate by the Booroola gene. Three Booroola genotypes (FecBFecB, FecBFec+ and Fec+Fec+) of the F2 population, from a cross between Booroola Merino and Scottish Blackface, and two Booroola genotypes (FecBFec+ and Fec+Fec+; 25% Booroola Merino and 75% Scottish Blackface), from the backcross of FecBFec+ sires to Scottish Blackface ewes, were compared. During seasonal anoestrus significant differences (P < 0.05) in hCG-stimulated ovulation rates were obtained between FecBFecB and Fec+Fec+ ewes from the F2 population, and FecBFec+ ewes were intermediate. No significant difference in hCG-stimulated ovulation rate was observed in the backcross population between FecBFec+ ewes and Fec+Fec+ ewes. There were no significant differences between genotypes in mean serum FSH concentrations during seasonal anoestrus in either backcross of F2 population. During the breeding season, two separate experiments confirmed the expected ovulation rate differences between genotypes (FecBFecB > FecBFec+ > Fec+Fec+). In both experiments, mean peripheral FSH concentrations in the F2 population were similar in FecBFec+ and Fec+Fec+ ewes, but were significantly higher (P < 0.05) in FecBFecB ewes. In the backcross population, mean peripheral FSH concentrations during the oestrous cycle were not significantly different between FecBFec+ and Fec+Fec+ ewes, despite significant differences in ovulation rate. Ovariectomy during the breeding season resulted in significantly higher (P < 0.001) mean peripheral FSH concentrations in all three genotypes. After ovariectomy, mean FSH concentrations between FecBFec+ and Fec+Fec+ ewes, form both backcross and F2 populations, were not significantly different.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究的目的是调查促卵泡素（FSH）在Booroola基因控制排卵率方面的作用。对Booroola美利奴羊与苏格兰黑脸羊杂交产生的F2代群体中的三种Booroola基因型（FecBFecB、FecBFec+和Fec+Fec+），以及FecBFec+公羊与苏格兰黑脸母羊回交产生的两种Booroola基因型（FecBFec+和Fec+Fec+；25%Booroola美利奴羊和75%苏格兰黑脸羊）进行了比较。在季节性乏情期，F2代群体中FecBFecB母羊与Fec+Fec+母羊经人绒毛膜促性腺激素（hCG）刺激后的排卵率存在显著差异（P<0.05），FecBFec+母羊的排卵率介于两者之间。在回交群体中，FecBFec+母羊与Fec+Fec+母羊经hCG刺激后的排卵率无显著差异。在F2代群体或回交群体的季节性乏情期，各基因型之间的平均血清FSH浓度均无显著差异。在繁殖季节，两项独立实验证实了各基因型之间预期的排卵率差异（FecBFecB>FecBFec+>Fec+Fec+）。在两项实验中，F2代群体中FecBFec+母羊和Fec+Fec+母羊的外周血FSH平均浓度相似，但FecBFecB母羊的浓度显著更高（P<0.05）。在回交群体中，尽管排卵率存在显著差异，但在发情周期中FecBFec+母羊与Fec+Fec+母羊的外周血FSH平均浓度无显著差异。在繁殖季节进行卵巢切除术后，所有三种基因型的外周血FSH平均浓度均显著升高（P<0.001）。卵巢切除术后，回交群体和F2代群体中FecBFec+母羊与Fec+Fec+母羊之间的FSH平均浓度无显著差异。（摘要截断于250字）
