Golenda C F, Klein T, Coleman R, Burge R, Ward R A, Seeley D C
Department of Entomology, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
J Med Entomol. 1995 May;32(3):300-5. doi: 10.1093/jmedent/32.3.300.
Reduction in total salivary gland protein from four anopheline vectors of human malaria, Anopheles stephensi Liston, An. albimanus Wiedmann, An. gambiae Giles, and An. freeborni Aitken, was quantified after mosquitoes blood-fed to repletion on human volunteers, hamsters or through a Baudruche artificial membrane. Total salivary gland protein from pools of six unfed mosquitoes ranged from 4.33 to 7.91 micrograms/ml. The difference between the total protein of glands from unfed and blood-fed mosquitoes for all species ranged from 1.77 to 3.12 (micrograms/ml for six pooled salivary glands. Total salivary gland protein for mosquitoes blood-fed to repletion was significantly less than that of unfed controls from the same cohort. Reduction in total salivary gland protein for An. freeborni and An stephensi blood fed to repletion on human volunteers, hamsters, and a Baudruche membrane ranged from 24 to 46%, from 43 to 56%, and from 24 to 51%, respectively. An. stephensi mosquitoes were allowed to blood feed on humans for 0 (unfed), 0.5-, 1.0-, 2.0-min time periods or to repletion (> 2-5 min). As feeding time increased, there was a significant decrease in total amount of protein in the salivary glands. This decrease was proportional over time, indicating that salivation occurred continuously from the beginning (probing) of blood feeding to withdrawal of the mosquito mouthparts at repletion. These data indicate that during blood feeding there difference between species in the salivary gland output measured as amount of protein depleted from the salivary glands and that depletion of salivary protein from the glands occurred continuously as mosquitoes fed to repletion.
在按蚊吸食人类志愿者、仓鼠的血液直至饱腹，或通过 Baudruche 人工膜吸食血液后，对四种传播人类疟疾的按蚊媒介，即斯氏按蚊（Anopheles stephensi Liston）、白纹按蚊（An. albimanus Wiedmann）、冈比亚按蚊（An. gambiae Giles）和费氏按蚊（An. freeborni Aitken）的唾液腺总蛋白减少量进行了量化。六只未进食蚊子的唾液腺总蛋白含量在 4.33 至 7.91 微克/毫升之间。所有物种未进食和进食后蚊子唾液腺总蛋白的差异在 1.77 至 3.12（六只唾液腺合并后的微克/毫升）之间。进食至饱腹的蚊子唾液腺总蛋白显著低于同一组未进食的对照。费氏按蚊和斯氏按蚊在吸食人类志愿者、仓鼠的血液直至饱腹以及通过 Baudruche 膜进食后，唾液腺总蛋白的减少量分别在 24%至 46%、43%至 56%和 24%至 51%之间。让斯氏按蚊在人类身上进食 0（未进食）、0.5、1.0、2.0 分钟时间段或直至饱腹（>2 - 5 分钟）。随着进食时间增加，唾液腺中蛋白总量显著减少。这种减少随时间成比例，表明从开始吸血（探查）到蚊子饱腹时口器撤出，唾液分泌持续进行。这些数据表明，在吸血过程中，以唾液腺中消耗的蛋白量衡量的唾液腺输出存在物种差异，并且随着蚊子进食至饱腹，唾液腺中的唾液蛋白持续消耗。
