Schengrund C L, Chi X, Sabol J, Griffith J W
Department of Biological Chemistry, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033, USA.
Lung. 1995;173(3):197-208. doi: 10.1007/BF00175660.
Experiments were carried out to determine the long-term effect of instillation of 500 mg of generic bituminous, anthracite, quartz, or titanium dioxide (TiO2) dust on the composition of pulmonary surfactant. Dust was instilled in the caudal lobe of the right lungs of female pigtailed macaque monkeys (Macaca nemestrina). The composition of surfactant isolated from cell-free bronchoalveolar lavage (CF-BAL) samples obtained from right lungs (dust exposed) at various times over the following year was compared with that of surfactant isolated from CF-BAL from left lungs (dust free). Little change was seen in the amount of surfactant-associated lipid phosphorus as a result of exposure to dust. Exposure to quartz, anthracite, or TiO2 dust induced a significant increase in the total amount of protein in the surfactant-enriched fraction. The relative amount of specific proteins was also altered: surfactant-associated protein A decreased, and the amount of the heavy and light chains of immunoglobulin molecules (identified by NH2-terminal amino acid sequence analysis) increased. These changes were visible more than a year after instillation of quartz and at least 3 months after instillation of anthracite dust. Despite variation in the responses of the individual animals, the changes observed might serve as an indicator of the severity of the effect of exposure of the lung to mineral dust and/or to pathogens.
进行了实验，以确定向雌性豚尾猕猴（食蟹猴）右肺尾叶滴注500毫克普通沥青、无烟煤、石英或二氧化钛（TiO₂）粉尘对肺表面活性剂成分的长期影响。将粉尘滴注到右肺（暴露于粉尘）的无细胞支气管肺泡灌洗（CF-BAL）样本中，在接下来的一年中，在不同时间从右肺获得的样本与从左肺（无粉尘）获得的CF-BAL中分离出的表面活性剂进行比较。由于接触粉尘，表面活性剂相关脂质磷的量几乎没有变化。接触石英、无烟煤或TiO₂粉尘会导致富含表面活性剂的部分中蛋白质总量显著增加。特定蛋白质的相对量也发生了变化：表面活性剂相关蛋白A减少，免疫球蛋白分子重链和轻链的量（通过NH₂末端氨基酸序列分析确定）增加。这些变化在滴注石英一年多后以及滴注无烟煤粉尘至少3个月后可见。尽管个体动物的反应存在差异，但观察到的变化可能作为肺部接触矿物粉尘和/或病原体影响严重程度的指标。
