Yélamos J, Klix N, Goyenechea B, Lozano F, Chui Y L, González Fernández A, Pannell R, Neuberger M S, Milstein C
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
Nature. 1995 Jul 20;376(6537):225-9. doi: 10.1038/376225a0.
Affinity maturation of antibodies is characterized by localized hypermutation of the DNA around the V segment. Here we show, using mice containing single or multiple transgene constructs, that an immunoglobulin V kappa segment can be replaced by human beta-globin or prokaryotic neo or gpt genes without affecting the rate of hypermutation; the V gene itself is not necessary for recruiting hypermutation. The ability to target hypermutation to heterologous genes in vivo could find more general applications in biology.
抗体的亲和力成熟以V基因片段周围DNA的局部超突变特征。在此，我们利用含有单个或多个转基因构建体的小鼠表明，免疫球蛋白Vκ基因片段可被人β-珠蛋白、原核neo或gpt基因取代，而不影响超突变率；V基因本身对于募集超突变并非必需。在体内将超突变靶向异源基因的能力可能在生物学中有更广泛的应用。
