Endo T, Sakata M
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Japan.
Pharmacol Toxicol. 1995 Mar;76(3):190-5. doi: 10.1111/j.1600-0773.1995.tb00128.x.
The effects of sulfhydryl compounds on the accumulation, removal and cytotoxicity of inorganic mercury (Hg) were investigated in primary cultures of rat renal cortical epithelial cells. The compounds investigated were 2,3-dimercaptosuccinic acid, 2,3-dimercapto-1-propanol, D-penicillamine, glutathione (GSH) and L-cysteine. In the accumulation experiment, the cells were co-incubated with Hg and the above compounds for 30 min. (short-term) or 18 hr (long-term). In the removal experiment, cells incubated with Hg were further incubated with the above compounds for 30 min. In both experiments, the alleviative effect of the compounds on the cytotoxicity was estimated by the uptake of neutral red or by cell growth. 2,3-Dimercaptosuccinic acid had the highest antidotal effects except for Hg removal. 2,3-Dimercapto-1-propanol exerted the least antidotal effects in the short-term, as well as in the long-term experiments, 2,3-dimercapto-1-propanol increased the Hg accumulation and the cytotoxicity despite its removal of most of the Hg. Although D-penicillamine, L-cysteine and GSH did not increase the Hg removal in the long-term experiment other antidotal effects were seen.
在大鼠肾皮质上皮细胞原代培养物中，研究了巯基化合物对无机汞（Hg）蓄积、清除及细胞毒性的影响。所研究的化合物有2,3-二巯基丁二酸、2,3-二巯基-1-丙醇、D-青霉胺、谷胱甘肽（GSH）和L-半胱氨酸。在蓄积实验中，细胞与汞及上述化合物共同孵育30分钟（短期）或18小时（长期）。在清除实验中，用汞孵育的细胞再与上述化合物孵育30分钟。在这两个实验中，通过中性红摄取或细胞生长来评估化合物对细胞毒性的缓解作用。除汞清除外，2,3-二巯基丁二酸具有最高的解毒效果。在短期和长期实验中，2,3-二巯基-1-丙醇的解毒效果最差，尽管它能清除大部分汞，但却增加了汞的蓄积和细胞毒性。虽然在长期实验中，D-青霉胺、L-半胱氨酸和GSH并未增加汞的清除，但可见其他解毒作用。
