Ghanta V K, Hiramoto N S, Soong S J, Hiramoto R N
Department of Microbiology, University of Alabama at Birmingham, 35294-0007.
Pharmacol Biochem Behav. 1995 Mar;50(3):399-403. doi: 10.1016/0091-3057(94)00286-r.
Studies from our laboratory demonstrated that conditioned resistance to the syngeneic YC8 lymphoma was established by multiple conditioned stimulus (CS)/unconditioned stimulus (US) associations. The conditioned stimulus used was exposure to the odor of camphor for 1 h and the unconditioned stimulus was an injection of DBA/2 spleen cell alloantigen that shares minor histocompatibility determinants with the YC8 lymphoma. To demonstrate a cellular basis for immune resistance to the YC8 tumor, BALB/c mice primed with DBA/2 spleen cell alloantigen were conditioned using a single trial CS/US association paradigm. Conditioned animals showed a measurable conditioned elevation of the cytotoxic T-lymphocyte (CTL) response to the YC8 tumor. Control groups in which the CS and US were not given in the proper sequence were unable to mount a conditioned response. These studies show that a secondary CTL response can be upregulated by the central nervous system (CNS).
我们实验室的研究表明，通过多次条件刺激（CS）/非条件刺激（US）联合，可建立对同基因YC8淋巴瘤的条件性抗性。所用的条件刺激是暴露于樟脑气味1小时，非条件刺激是注射与YC8淋巴瘤共享次要组织相容性决定簇的DBA/2脾细胞同种异体抗原。为了证明对YC8肿瘤免疫抗性的细胞基础，用DBA/2脾细胞同种异体抗原致敏的BALB/c小鼠采用单次试验CS/US联合范式进行条件训练。经条件训练的动物对YC8肿瘤的细胞毒性T淋巴细胞（CTL）反应出现可测量的条件性增强。对照组中CS和US未按正确顺序给予，无法产生条件性反应。这些研究表明，中枢神经系统（CNS）可上调继发性CTL反应。
