Ghanta V K, Hiramoto N S, Solvason H B, Soong S J, Hiramoto R N
Department of Biology, University of Alabama, Birmingham 35294.
Cancer Res. 1990 Jul 15;50(14):4295-9.
It has been demonstrated by Parmiani et al. (Int. J. Cancer, 29: 323-332, 1982) that a significant protective effect can be obtained against the transplanted syngeneic YC8 lymphoma by prior immunization of BALB/c mice with normal allogeneic DBA/2 spleen cells. Using this well established tumor model, we investigated a novel approach, conditioning of specific immunotherapeutic activity. For this purpose, we used the odor of camphor as the conditioning stimulus and allogeneic DBA/2 spleen cells as unconditioning stimulus. We associated the conditioning and unconditioning stimuli two, three, and four times. Following this the conditioned animals were reexposed to the odor of camphor only. In each case, we observed a delay in tumor growth and in some instances the conditioned group performed better than the immunotherapy control group. These results indicate that a limited number of treatments with the antigen is better than the continuous treatment in maintaining the immunity and the homeostasis of the system.
帕尔米阿尼等人（《国际癌症杂志》，第29卷，第323 - 332页，1982年）已证明，通过用正常同种异体DBA/2脾细胞预先免疫BALB/c小鼠，可对移植的同基因YC8淋巴瘤产生显著的保护作用。利用这个成熟的肿瘤模型，我们研究了一种新方法，即调节特异性免疫治疗活性。为此，我们使用樟脑气味作为条件刺激物，同种异体DBA/2脾细胞作为非条件刺激物。我们将条件刺激物和非条件刺激物关联了2次、3次和4次。在此之后，仅让经条件反射的动物再次接触樟脑气味。在每种情况下，我们都观察到肿瘤生长延迟，并且在某些情况下，经条件反射的组比免疫治疗对照组表现更好。这些结果表明，在维持系统的免疫力和内环境稳定方面，有限次数的抗原治疗比持续治疗更好。
