Park H J, Kim H N
Department of Otolaryngology, Ajou University School of Medicine, Suwon, Korea.
Yonsei Med J. 1995 May;36(2):111-5. doi: 10.3349/ymj.1995.36.2.111.
The central projections of the peripheral cochlear nerve fiber from each turn to the cochlear nuclei (CN) in the mongolian gerbil were investigated using retrograde transportation of horseradish peroxidase (HRP). The organ of Corti and the osseous spiral lamina were scratched with an electrolytically-sharpened fine needle via a small hole at each turn of the cochlea. The cochlea was filled with a 30% horseradish peroxidase (HRP) solution. After 48 hours, 50 microns transverse serial sections of the brainstem were made with a vibratome. The tissue was processed with the diaminobenzidine procedure of the cobalt-glucose method. Our experiment revealed that the fibers from the basal turn terminated at the dorsomedial portion of anteroventral cochlear nuclei (AVCN), but those from the apical turn were distributed among the ventrolateral portion of the AVCN. In the posteroventral cochlear nuclei (PVCN) and dorsal cochlear nuclei (DCN), the fibers from basal to apical turns extend from the dorsal to the ventral portion of each nuclei. A distinct tonotopic arrangement could be found between the origin of cochlear fibers of each turn and their termination in the regions of the cochlear nuclei (CN). Also, the results suggested that the scratch method combined with retrograde transportation of horseradish peroxidase was useful in investigating the tonotopic arrangement of the peripheral auditory nerve in the CN.
利用辣根过氧化物酶（HRP）逆行运输法，研究了蒙古沙鼠中从每一圈外周耳蜗神经纤维到耳蜗核（CN）的中枢投射。通过耳蜗每一圈的小孔，用经电解锐化的细针刮擦柯蒂氏器和骨螺旋板。向耳蜗内注入30%的辣根过氧化物酶（HRP）溶液。48小时后，用振动切片机制作脑干的50微米横向连续切片。采用钴-葡萄糖法的二氨基联苯胺程序对组织进行处理。我们的实验表明，来自基底部的纤维终止于前腹侧耳蜗核（AVCN）的背内侧部分，但来自顶部的纤维分布于AVCN的腹外侧部分。在蜗背侧核（DCN）和蜗后腹侧核（PVCN）中，从基底部到顶部的纤维从每个核团的背侧延伸至腹侧。在每一圈耳蜗纤维的起源与其在耳蜗核（CN）区域的终止之间，可以发现明显的音调定位排列。此外，结果表明，刮擦法结合辣根过氧化物酶逆行运输法有助于研究CN中外周听神经的音调定位排列。
