开发一种基于单克隆抗体的免疫分析法，用于检测和定量定殖在叶片材料上的长孢鳝孢霉。
Development of a monoclonal antibody-based immunoassay for the detection and quantification of Anguillospora longissima colonizing leaf material.
作者信息
Bermingham S, Dewey F M, Maltby L
机构信息
Department of Plant Sciences, University of Oxford, United Kingdom.
出版信息
Appl Environ Microbiol. 1995 Jul;61(7):2606-13. doi: 10.1128/aem.61.7.2606-2613.1995.
Monoclonal antibodies (MAbs) to the aquatic hyphomycete Anguillospora longissima were raised in mice by using a coimmunization program. A cell line was raised that produced a MAb of the immunoglobulin M class that was specific for A. longissima both in an enzyme-linked immunosorbent assay (ELISA) and by immunofluorescence but that did not recognize other members of the aquatic hyphomycetes. This MAb (AL-HH8c) was used to develop a quantitative ELISA in vitro. The antigen recognized by AL-HH8c is produced throughout the mycelium, irrespective of mycelial age and culture conditions. By using this MAb, mycelium of A. longissima colonizing leaf material can be detected by ELISA, immunofluorescence, and immunoenzymatic staining methods.
通过共同免疫程序在小鼠体内产生了针对水生真菌长蠕孢菌（Anguillospora longissima）的单克隆抗体（MAb）。建立了一个细胞系，该细胞系产生了免疫球蛋白M类的单克隆抗体，在酶联免疫吸附测定（ELISA）和免疫荧光检测中对长蠕孢菌具有特异性，但不识别其他水生真菌成员。这种单克隆抗体（AL-HH8c）被用于开发一种体外定量ELISA。AL-HH8c识别的抗原在整个菌丝体中产生，与菌丝体的年龄和培养条件无关。通过使用这种单克隆抗体，可以通过ELISA、免疫荧光和免疫酶染色方法检测定殖在叶片材料上的长蠕孢菌的菌丝体。
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