利用聚合酶链式反应（PCR）检测不同植物物种根系中的丛枝菌根真菌。
Detection of an arbuscular mycorrhizal fungus in roots of different plant species with the PCR.
作者信息
Di Bonito R, Elliott M L, Des Jardin E A
机构信息
INN-BIOAG, ENEA CRE Casaccia, Rome, Italy.
出版信息
Appl Environ Microbiol. 1995 Jul;61(7):2809-10. doi: 10.1128/aem.61.7.2809-2810.1995.
Abstract
PCR was used with the primer pair VANS1-NS21 to detect the arbuscular mycorrhizal fungus Glomus intraradices (commercial inoculum source) on roots of lettuce, zinnia, leek, pepper, and endive plants. The appropriate amplification product was obtained directly from roots without DNA extraction and purification.
摘要
使用引物对VANS1-NS21进行聚合酶链反应（PCR），以检测生菜、百日草、韭菜、辣椒和菊苣植株根系上的丛枝菌根真菌根内球囊霉（商业接种源）。无需进行DNA提取和纯化，直接从根系中获得了合适的扩增产物。
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