利用聚合酶链式反应（PCR）检测不同植物物种根系中的丛枝菌根真菌。
Detection of an arbuscular mycorrhizal fungus in roots of different plant species with the PCR.
作者信息
Di Bonito R, Elliott M L, Des Jardin E A
机构信息
INN-BIOAG, ENEA CRE Casaccia, Rome, Italy.
出版信息
Appl Environ Microbiol. 1995 Jul;61(7):2809-10. doi: 10.1128/aem.61.7.2809-2810.1995.
Abstract
PCR was used with the primer pair VANS1-NS21 to detect the arbuscular mycorrhizal fungus Glomus intraradices (commercial inoculum source) on roots of lettuce, zinnia, leek, pepper, and endive plants. The appropriate amplification product was obtained directly from roots without DNA extraction and purification.
摘要
使用引物对VANS1-NS21进行聚合酶链反应（PCR），以检测生菜、百日草、韭菜、辣椒和菊苣植株根系上的丛枝菌根真菌根内球囊霉（商业接种源）。无需进行DNA提取和纯化，直接从根系中获得了合适的扩增产物。
相似文献
1
Detection of an arbuscular mycorrhizal fungus in roots of different plant species with the PCR.利用聚合酶链式反应（PCR）检测不同植物物种根系中的丛枝菌根真菌。
Appl Environ Microbiol. 1995 Jul;61(7):2809-10. doi: 10.1128/aem.61.7.2809-2810.1995.
2
Rapid quantitation by PCR of endomycorrhizal fungi colonizing roots.通过聚合酶链式反应（PCR）对定殖于根部的内生菌根真菌进行快速定量分析。
PCR Methods Appl. 1992 Aug;2(1):76-80. doi: 10.1101/gr.2.1.76.
3
Characterization of root colonization profiles by a microcosm community of arbuscular mycorrhizal fungi using 25S rDNA-targeted nested PCR.利用靶向25S rDNA的巢式PCR对丛枝菌根真菌微观群落的根系定殖情况进行表征。
Mol Ecol. 1998 Jul;7(7):879-87. doi: 10.1046/j.1365-294x.1998.00410.x.
4
Generation of RAPD-PCR primers for the identification of isolates of Glomus mosseae, an arbuscular mycorrhizal fungus.用于鉴定丛枝菌根真菌摩西球囊霉分离株的随机扩增多态性PCR引物的生成
Mol Ecol. 1995 Feb;4(1):61-8. doi: 10.1111/j.1365-294x.1995.tb00192.x.
5
Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots.从定殖于根部的泡囊-丛枝内生菌根真菌中特异性扩增18S真菌核糖体基因。
Appl Environ Microbiol. 1992 Jan;58(1):291-5. doi: 10.1128/aem.58.1.291-295.1992.
6
Characterization of a highly repeated DNA sequence (SC1) from the arbuscular mycorrhizal fungus Scutellospora castanea and its detection in planta.来自丛枝菌根真菌栗色盾孢囊霉的一个高度重复DNA序列（SC1）的特性及其在植物中的检测
Appl Environ Microbiol. 1996 Jul;62(7):2443-8. doi: 10.1128/aem.62.7.2443-2448.1996.
7
Direct quantification of fungal DNA from soil substrate using real-time PCR.
J Microbiol Methods. 2003 Apr;53(1):67-76. doi: 10.1016/s0167-7012(02)00225-7.
8
Ribosomal small subunit sequence variation within spores of an arbuscular mycorrhizal fungus, Scutellospora sp.丛枝菌根真菌盾孢囊霉属（Scutellospora sp.）孢子内核糖体小亚基序列变异
Mol Ecol. 1999 Jun;8(6):915-21. doi: 10.1046/j.1365-294x.1999.00642.x.
9
Taxon-specific oligonucleotide primers for detection of two ancient endomycorrhizal fungi, Glomus occultum and Glomus brasilianum.用于检测两种古老的内生菌根真菌——隐匿球囊霉和巴西球囊霉的分类群特异性寡核苷酸引物。
FEMS Microbiol Lett. 2001 Mar 15;196(2):165-70. doi: 10.1111/j.1574-6968.2001.tb10559.x.
10
Nested multiplex PCR--a feasible technique to study partial community of arbuscular mycorrhizal fungi in field-growing plant root.巢式多重聚合酶链反应——一种研究田间生长植物根系丛枝菌根真菌部分群落的可行技术。
Sci China C Life Sci. 2006 Aug;49(4):354-61. doi: 10.1007/s11427-006-2008-z.
引用本文的文献
1
a new species from Western North America.一种来自北美西部的新物种。
Fungal Syst Evol. 2024 Dec;14:191-196. doi: 10.3114/fuse.2024.14.12. Epub 2024 Jun 7.
2
Recent Progress, Challenges, and Prospects in Two-Dimensional Photo-Catalyst Materials and Environmental Remediation.二维光催化材料与环境修复的研究进展、挑战及前景
Nanomicro Lett. 2020 Aug 15;12(1):167. doi: 10.1007/s40820-020-00504-3.
3
Rapid and reliable DNA extraction techniques from trypan-blue-stained mycorrhizal roots: comparison of two methods.从经台盼蓝染色的菌根根中快速可靠地提取DNA的技术：两种方法的比较。
Mycorrhiza. 2004 Aug;14(4):271-5. doi: 10.1007/s00572-004-0316-3. Epub 2004 Jun 25.
4
Defoliation effects on the community structure of arbuscular mycorrhizal fungi based on 18S rDNA sequences.基于18S rDNA序列的落叶对丛枝菌根真菌群落结构的影响
Mycorrhiza. 2004 Dec;14(6):363-73. doi: 10.1007/s00572-003-0286-x. Epub 2004 Jan 15.
5
Fot 1 insertions in the Fusarium oxysporum f. sp. albedinis genome provide diagnostic PCR targets for detection of the date palm pathogen.尖孢镰刀菌古巴专化型基因组中的Fot 1插入序列为检测枣椰树病原体提供了诊断性PCR靶点。
Appl Environ Microbiol. 1998 Feb;64(2):633-6. doi: 10.1128/AEM.64.2.633-636.1998.
本文引用的文献
1
Identification of endomycorrhizal fungi colonizing roots by fluorescent single-strand conformation polymorphism-polymerase chain reaction.通过荧光单链构象多态性-聚合酶链反应鉴定定殖于根部的内生菌根真菌
Appl Environ Microbiol. 1993 Dec;59(12):4211-5. doi: 10.1128/aem.59.12.4211-4215.1993.
2
The effects of plant polysaccharides and buffer additives on PCR.植物多糖和缓冲添加剂对聚合酶链式反应的影响。
Biotechniques. 1992 Mar;12(3):332-4.
3
Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction.用于聚合酶链反应的从沉积物腐殖质中分离细菌DNA的快速方法
Appl Environ Microbiol. 1992 Jul;58(7):2292-5. doi: 10.1128/aem.58.7.2292-2295.1992.
4
An efficient method for isolation of RNA and DNA from plants containing polyphenolics.一种从含多酚类物质的植物中分离RNA和DNA的有效方法。
Nucleic Acids Res. 1992 May 11;20(9):2381. doi: 10.1093/nar/20.9.2381.
5
Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots.从定殖于根部的泡囊-丛枝内生菌根真菌中特异性扩增18S真菌核糖体基因。
Appl Environ Microbiol. 1992 Jan;58(1):291-5. doi: 10.1128/aem.58.1.291-295.1992.
6
Rapid quantitation by PCR of endomycorrhizal fungi colonizing roots.通过聚合酶链式反应（PCR）对定殖于根部的内生菌根真菌进行快速定量分析。
PCR Methods Appl. 1992 Aug;2(1):76-80. doi: 10.1101/gr.2.1.76.
Zotero 插件