Zeze A, Hosny M, Gianinazzi-Pearson V, Dulieu H
Laboratoire de Phytoparasitologie INRA-CNRS, Station de Génétique et d'Amélioration des Plantes, INRA, Dijon, France.
Appl Environ Microbiol. 1996 Jul;62(7):2443-8. doi: 10.1128/aem.62.7.2443-2448.1996.
A highly repeated DNA sequence from the genome of an arbuscular mycorrhizal fungus has been isolated and characterized. This 1,202-bp sequence (SC1) represents about 0.24% of the Scutellospora castanea genome, estimated to be 1 pg by flow cytometry. The sequence was shown to be a Scutellospora-specific probe in Southern blots and dot blot hybridizations. After complete sequencing of SC1, PCR primers were generated and used to amplify a 907-bp fragment from spores of S. castanea or from colonized Allium porrum roots. No amplification products were obtained with DNA from either spores or mycorrhizal root of other species of arbuscular mycorrhizal fungi. These primers were sufficiently specific for unequivocal detection of S. castanea in planta.
一种来自丛枝菌根真菌基因组的高度重复DNA序列已被分离和鉴定。这个1202碱基对的序列（SC1）约占栗色盾孢囊霉基因组的0.24%，通过流式细胞术估计该基因组大小为1皮克。在Southern印迹和斑点杂交中，该序列被证明是栗色盾孢囊霉特异性探针。对SC1进行全序列测定后，设计了PCR引物，并用于从栗色盾孢囊霉的孢子或定殖的葱根中扩增出一个907碱基对的片段。从其他丛枝菌根真菌的孢子或菌根根中提取的DNA未获得扩增产物。这些引物具有足够的特异性，能够在植物中明确检测到栗色盾孢囊霉。
