Watanabe Y, Suzui J, Ichikawa Y, Yoshida K, Suzuki H
Department of Pediatrics, Fukushima Medical College.
Arerugi. 1995 May;44(5):547-55.
To confirm that the soluble factors produced by peripheral blood mononuclear cells (PBMC) from children with minimal change nephrotic syndrome (MCNS) enhance thromboxane (TX) A2 synthesis, we studied the urinary albumin and TXA2 metabolite excretions of rats intravenously injected with PBMC culture supernatant (PCS). Furthermore, we studied which does mainly effect on urinary albumin excretion, TXA2 metabolism in kidney or in platelet. In this study, the urinary albumin, TXB2, and 11-dehydro-TXB2 excretions of rats injected with PCS from MCNS children were remarkably increased, but this change was not observed in rats injected with PCS from children with MCNS in remission, glomerulonephritis, or healthy controls. The urinary albumin excretion of rats administered with acetylsalitylic acid before injection of PCS from MCNS children did not increase. We concluded that the soluble factors produced by PBMC from MCNS children increase urinary albumin excretion, and that the TXA2 metabolism is closely related to this effect.
为了证实微小病变型肾病综合征（MCNS）患儿外周血单个核细胞（PBMC）产生的可溶性因子可增强血栓素（TX）A2的合成，我们研究了静脉注射PBMC培养上清液（PCS）的大鼠的尿白蛋白和TX A2代谢产物排泄情况。此外，我们研究了主要是肾脏还是血小板中的TX A2代谢对尿白蛋白排泄有影响。在本研究中，注射MCNS患儿PCS的大鼠的尿白蛋白、TXB2和11-脱氢-TXB2排泄显著增加，但在注射缓解期MCNS患儿、肾小球肾炎患儿或健康对照者PCS的大鼠中未观察到这种变化。在注射MCNS患儿PCS前给予乙酰水杨酸的大鼠，其尿白蛋白排泄未增加。我们得出结论，MCNS患儿PBMC产生的可溶性因子增加尿白蛋白排泄，且TX A2代谢与此效应密切相关。
