Wendt C, Weist K, Dietz E, Schlattmann P, Rüden H
Nationales Referenzzentrum für Krankenhaushygiene, Freien Universität Berlin.
Zentralbl Hyg Umweltmed. 1995 Mar;196(6):515-31.
The efficiency of filters for elimination of Legionella was examined in a ten month field study. Showers and sinks of twelve patient rooms were equipped with nylon 66-polyamid filters. Before filtration the total number of colonies was very high with an arithmetical mean of 1131 cfu/ml. This could be reduced by filtration at the sinks (mean = 205 cfu/ml). After equipping showers with filters we found a significant increase in the total number of colonies (mean = 1961 cfu/ml). In non-filtered water we found Legionella in nearly 90% of the 648 samples, the concentration extended to 300 cfu/ml (mean = 51 cfu/ml). After filtration the concentration of Legionella was up to 280 cfu/ml in 7.3% of the 665 samples. We calculated a logistic regression model to examine the effects of the age of the filters and the duration of their use since the last sterilization. These parameters were calculated from the time of the break through or retrograde contamination with Legionella. There were clear differences between shower and sink filters. Assuming a 5% probability of break through or retrograde contamination with Legionella, the sink filters should be sterilized every 3 weeks without regards to the age of the filters. In contrast the shower filters may be used for 3 weeks if they are sterilized weekly. If weekly sterilization is not feasible they should be used for 2 weeks only. In our study it was not possible to achieve Legionella-free water by changing either the frequency of sterilization or the age of the filters.
在一项为期十个月的实地研究中,对用于消除军团菌的过滤器效率进行了检测。十二个病房的淋浴喷头和水槽都配备了尼龙66聚酰胺过滤器。过滤前菌落总数非常高,算术平均值为1131 cfu/ml。水槽过滤后这一数值可降低(平均值 = 205 cfu/ml)。在淋浴喷头配备过滤器后,我们发现菌落总数显著增加(平均值 = 1961 cfu/ml)。在未过滤的水中,我们在648个样本中近90%检测到军团菌,浓度高达300 cfu/ml(平均值 = 51 cfu/ml)。过滤后,在665个样本中有7.3%的样本中军团菌浓度高达280 cfu/ml。我们计算了一个逻辑回归模型,以检测过滤器使用时长及其自上次灭菌后使用时间对结果的影响。这些参数是根据军团菌突破或逆行污染的时间计算得出的。淋浴喷头过滤器和水槽过滤器之间存在明显差异。假设军团菌突破或逆行污染的概率为5%,水槽过滤器应每3周进行一次灭菌,无需考虑过滤器的使用时长。相比之下,淋浴喷头过滤器如果每周进行灭菌,则可使用3周。如果无法每周进行灭菌,则只能使用2周。在我们的研究中,无论是改变灭菌频率还是过滤器使用时长,都无法实现无军团菌水。