Cable J, Jackson I J, Steel K P
MRC Institute of Hearing Research, University Park, Nottingham, UK.
Mech Dev. 1995 Apr;50(2-3):139-50. doi: 10.1016/0925-4773(94)00331-g.
The development of melanoblasts in normally pigmented and dominant spotting (W) embryos was followed by in situ hybridisation to TRP-2/DT mRNA, which labels migratory melanoblasts from 10 days post coitum. Numerous melanoblasts migrate to the inner ear around 11 days. In contrast, few migratory melanoblasts are associated with the eye or skin at this stage and melanoblasts distribution within the trunk and tail is patchy. The distribution of melanoblasts in 10.5-11-day-old Wv/Wv, Wsh/Wsh and W41/W41 mutants was similar to that in controls but melanoblasts density was lower and by 12 days was severely reduced. These results suggest that mutations of the c-kit receptor tyrosine kinase encoded at the W locus do not alter early migration or differentiation of melanoblasts but severely affect melanoblasts survival.
通过对TRP-2/DT mRNA进行原位杂交，追踪正常色素沉着和显性斑点（W）胚胎中黑素母细胞的发育情况，该mRNA可标记交配后10天起的迁移性黑素母细胞。大约在11天时，大量黑素母细胞迁移至内耳。相比之下，此时与眼睛或皮肤相关的迁移性黑素母细胞很少，并且黑素母细胞在躯干和尾部的分布是不连续的。10.5 - 11日龄的Wv/Wv、Wsh/Wsh和W41/W41突变体中黑素母细胞的分布与对照相似，但黑素母细胞密度较低，到12天时严重降低。这些结果表明，位于W位点的c-kit受体酪氨酸激酶突变不会改变黑素母细胞的早期迁移或分化，但会严重影响黑素母细胞的存活。
