de la Peña L, Gómez-Hens A, Pérez-Bendito D
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
J Pharm Biomed Anal. 1995 Mar;13(3):199-203. doi: 10.1016/0731-7085(95)01267-o.
A fast kinetic method is proposed for the photometric and fluorimetric determination of nortriptyline hydrochloride. The method involves measuring the rate of formation of an adduct with 4-chloro-7-nitrobenzofurazan, which exhibits light absorption and fluorescent properties, and the use of a stopped-flow mixing technique, which facilitates application to automatic routine analyses. The reaction rate is measured within only 30 s. The detection limit is 0.12 micrograms ml-1 (photometry) and 0.18 micrograms ml-1 (fluorimetry) and the calibration graph is linear up to 60 micrograms ml-1 in both cases. The precision (as RSD) is less than 1.5%. The proposed method was satisfactorily used for direct analysis of pharmaceutical preparations and a mean recovery near 100% was obtained with both photometric and fluorimetric detection.
