de la Peña L, Gómez-Hens A, Pérez-Bendito D
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
J Pharm Biomed Anal. 1993 Oct;11(10):893-6. doi: 10.1016/0731-7085(93)80046-4.
The oxidation of promethazine to its corresponding fluorescent sulphoxide was used to develop a novel kinetic fluorimetric method for the determination of this drug. The use of a stopped-flow mixing technique makes use of an oxidizing reagent unnecessary because the oxidation is rapidly carried out by dissolved oxygen. The method is simple and fast as it only requires a few seconds to obtain kinetic data which allows ready application to routine analyses. The calibration graph is linear over the range 0.5-80 micrograms ml-1 and the precision (%RSD) is close to 2%. The method was applied to the determination of promethazine hydrochloride in two pharmaceutical preparations.
