Aihara N, Tazuma S, Kajiyama G
First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
J Gastroenterol Hepatol. 1995 Jan-Feb;10(1):42-6. doi: 10.1111/j.1440-1746.1995.tb01045.x.
Bile salts can be cytotoxic to mucosal surfaces, because of their detergent properties. This is not normally seen under physiological circumstances in the gall-bladder. To further study normal mucosal defence mechanisms, the present study was performed to examine the effects of liposomes and hydrophilic bile salts on hydrophobic bile salt-induced release of radiolabelled glycoproteins from explants of guinea-pig gall-bladder. Glycoprotein release was correlated with the degree of hydrophobicity of bile salts, as determined by the retention factor in reversed-phase high-performance liquid chromatography. Hydrophobic bile salt-induced release of glycoproteins was reduced by liposomes and hydrophilic bile salts. The inhibitory effect of liposomes was directly related to the degree of saturation of their fatty acyl chains, and that of hydrophilic bile salts was related to the degree of hydrophilicity. These findings suggest that vesicles and hydrophilic bile salts may play a cytoprotective role against membrane damage passively caused by hydrophobic bile salts in the biliary system, and that such damage may occur according to the quantitative and qualitative imbalance among these factors.
胆汁盐因其去污剂特性，可能对黏膜表面具有细胞毒性。在生理情况下，胆囊中通常不会出现这种情况。为了进一步研究正常的黏膜防御机制，本研究旨在检测脂质体和亲水性胆汁盐对疏水性胆汁盐诱导豚鼠胆囊外植体释放放射性标记糖蛋白的影响。糖蛋白的释放与胆汁盐的疏水性程度相关，疏水性程度通过反相高效液相色谱中的保留因子来确定。脂质体和亲水性胆汁盐可减少疏水性胆汁盐诱导的糖蛋白释放。脂质体的抑制作用与它们脂肪酰链的饱和度直接相关，亲水性胆汁盐的抑制作用与亲水性程度相关。这些发现表明，囊泡和亲水性胆汁盐可能对胆道系统中疏水性胆汁盐被动引起的膜损伤起到细胞保护作用，并且这种损伤可能根据这些因素之间的数量和质量失衡而发生。
