Ross P E, Butt A N, Gallacher C
Department of Medicine, Ninewells Hospital Medical School, Dundee.
J Clin Pathol. 1990 Jul;43(7):572-5. doi: 10.1136/jcp.43.7.572.
Model and real biles were used to investigate factors influencing cholesterol and dextran (70,000 molecular weight) absorption by the gall bladder. Cholesterol absorption was proportional to cholesterol concentration when real bile was used, but model biles showed maximal absorption at cholesterol saturation. Reduction of temperature reduced cholesterol absorption and serosal secretion, but had little effect on dextran absorption. This indicates differences in uptake where cholesterol undergoes passive diffusion but dextran is taken up by fluid-phase endocytosis. Model bile prepared with a single bile salt showed lowest cholesterol uptake from cholate bile, but there was no difference in serosal secretion. Dextran uptake was also lowest from cholate bile, although serosal secretion was highest. These results show that an increase in the biliary content of dihydroxy bile salts increases gall bladder permeability to both hydrophobic and hydrophilic molecules and may lead to the accumulation of lipids in the mucosa, as seen in cholesterolosis.
利用模型胆汁和真实胆汁研究影响胆囊对胆固醇和右旋糖酐（分子量70,000）吸收的因素。当使用真实胆汁时，胆固醇的吸收与胆固醇浓度成正比，但模型胆汁在胆固醇饱和时显示出最大吸收量。温度降低会减少胆固醇的吸收和浆膜分泌，但对右旋糖酐的吸收影响很小。这表明在摄取方面存在差异，其中胆固醇通过被动扩散吸收，而右旋糖酐通过液相内吞作用摄取。用单一胆盐制备的模型胆汁从胆酸盐胆汁中摄取的胆固醇最低，但浆膜分泌没有差异。尽管浆膜分泌最高，但胆酸盐胆汁中的右旋糖酐摄取也最低。这些结果表明，二羟基胆盐胆汁含量的增加会增加胆囊对疏水分子和亲水分子的通透性，并可能导致脂质在粘膜中积累，如在胆固醇沉着症中所见。
