Kawashima S, Imahori K
J Biochem. 1979 Jan;85(1):197-202. doi: 10.1093/oxfordjournals.jbchem.a132310.
Histone oligomers were reconstituted from calf thymus whole histones by annealing from 2 M NaCl-5 M urea-0.05 M Tris buffer (pH 8.0)-1% beta-mercaptoethanol, and were fractionated in terms of solubility in ammonium sulfate solution. Fractionation occurred according to the histone compositions of oligomers. The oligomers thus obtained were (1) pentamer and trimer of H1, (2) tetramer of H2A and H2B, and (3) tetramer and higher aggregates of H3 and H4, under the following ionic conditions: 0.5 M NaCl-0.05 M phosphate buffer (pH 7.0). The aggregates of H3 and H4 could easily be converted to the tetramer by lowering of pH.
通过在2M NaCl - 5M尿素 - 0.05M Tris缓冲液（pH 8.0）- 1%β-巯基乙醇中退火，从小牛胸腺全组蛋白中重构组蛋白寡聚体，并根据其在硫酸铵溶液中的溶解度进行分级分离。分级分离根据寡聚体的组蛋白组成进行。在以下离子条件下：0.5M NaCl - 0.05M磷酸盐缓冲液（pH 7.0），由此获得的寡聚体为：（1）H1的五聚体和三聚体，（2）H2A和H2B的四聚体，以及（3）H3和H4的四聚体及更高聚集体。通过降低pH值，H3和H4的聚集体可以很容易地转化为四聚体。
