Lindsey G G, Thompson P, Purves L R, von Holt C
FEBS Lett. 1982 Aug 16;145(1):131-6. doi: 10.1016/0014-5793(82)81221-0.
Simple mixing of acid purified histones H3 and H4 in equimolar quantities at low ionic strength near pH 7 does not yield the tetramer but rather a high Mr aggregate. Dialysis of acid extracted total or core histones into 2 M NaCl 150 mM phosphate (pH 7.4) followed by fractionation of the histone complexes at lower ionic strength (150 mM NaCl) results in an H3-H4 tetramer of a structure identical to that derived from salt-extracted histones. Dialysis of acid extracted total or core histones directly into the lower ionic strength buffer with subsequent fractionation, results in H3-H4 tetramer of closely similar structure.
在接近pH 7的低离子强度下，将酸纯化的组蛋白H3和H4以等摩尔量简单混合，不会产生四聚体，而是形成高分子量聚集体。将酸提取的总组蛋白或核心组蛋白透析到2M NaCl、150mM磷酸盐（pH 7.4）中，然后在较低离子强度（150mM NaCl）下对组蛋白复合物进行分级分离，会得到结构与从盐提取组蛋白中得到的相同的H3-H4四聚体。将酸提取的总组蛋白或核心组蛋白直接透析到较低离子强度缓冲液中，随后进行分级分离，会得到结构非常相似的H3-H4四聚体。
