小亚基核糖体RNA中的一个三核苷酸特征序列将人体贾第虫分为两种不同的基因型。
A three nucleotide signature sequence in small subunit rRNA divides human Giardia in two different genotypes.
作者信息
van Keulen H, Homan W L, Erlandsen S L, Jarroll E L
机构信息
Department of Biology, Cleveland State University, Ohio 44115, USA.
出版信息
J Eukaryot Microbiol. 1995 Jul-Aug;42(4):392-4. doi: 10.1111/j.1550-7408.1995.tb01600.x.
The nucleotide sequence of the 16S rRNA gene and the space DNA region was determined for Giardia duodenalis, obtained from humans in The Netherlands (AMC-4) and Washington State (CM). These rDNA sequences differ from other G. duodenalis isolates (Portland-1 and BRIS/83/HEPU/ 106) both of which have virtually identical rDNA sequences. The most characteristic feature was found close to the 5' end of the 16S rRNA. The Portland-1 -Bris/83/HEPU/ 106 type has GCG in position 22-24, while AMC-4 and CM have AUC in this position. These two sequences, present in an otherwise conserved region of the 16S rRNA, are "signature" sequences, which divide Giardia isolates into two different groups.
测定了从荷兰（AMC - 4）和华盛顿州（CM）的人类体内分离出的十二指肠贾第虫16S rRNA基因和间隔DNA区域的核苷酸序列。这些核糖体DNA序列与其他十二指肠贾第虫分离株（波特兰 - 1和BRIS/83/HEPU/106）不同，后两者的核糖体DNA序列几乎相同。最显著的特征出现在16S rRNA的5'端附近。波特兰 - 1 - Bris/83/HEPU/106型在第22 - 24位是GCG，而AMC - 4和CM在该位置是AUC。这两个序列存在于16S rRNA的一个其他区域保守的区域中，是“特征”序列，它们将贾第虫分离株分为两个不同的组。
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